Microfluidically fabricated fibers containing pancreatic islets and mesenchymal stromal cells improve longevity and sustained normoglycemia in diabetic rats

Navaei-Nigjeh M, Mirzababaei S, Ghiass MA, Roshanbinfar K, Gholami M, Abdollahi M (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Biofabrication IOP Publishing

Book Volume: 15

Journal Issue: 1

DOI: 10.1088/1758-5090/ac9d04

Abstract

Type 1 diabetes mellitus is an autoimmune disease characterized by the loss of pancreatic isletβcells. Insulin injections and pancreas transplants are currently available therapies. The former requires daily insulin injections, while the latter is constrained by donor organ availability. Islet transplantation is a promising alternative treatment for type 1 diabetes mellitus that may overcome the limitations of previous techniques. Two challenges, however, must be addressed: limited cell retention as a result of the immune response and limited function of the transplanted cells that survive. To address these problems, we developed a microfluidic technology for a one-step generation of islet-laden fibers to protect them from the immune response. This approach enables continuous generation of microfibers with a diameter suitable for islet encapsulation (275µm). We, then, transplanted islet-laden fibers into diabetic Wistar rats. While islet-laden fibers alone were unable to restore normoglycemia in diabetic rats, adding mesenchymal stromal cells (MSCs) restored normoglycemia for an extended time. It increased the animals' lifespan by up to 75 d. Additionally, it improved the glucose-stimulated response of islets to the point where there was no significant difference between the treatment group and the healthy animals. Additionally, the presence of MSCs suppressed the immune response, as seen by decreased levels of pro-inflammatory cytokines such as tumor necrosis factor-α. Taken together, these fibers including islet and MSCs provide a versatile platform for concurrently improving cell preservation and functioning followingin vivotransplantation.

Authors with CRIS profile

Kaveh Roshanbinfar Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

Tehran University of Medical Sciences (TUMS) / دانشگاه علوم پزشکی تهران IR Iran, Islamic Republic of (IR) Tarbiat Modares University IR Iran, Islamic Republic of (IR)

How to cite

APA:

Navaei-Nigjeh, M., Mirzababaei, S., Ghiass, M.A., Roshanbinfar, K., Gholami, M., & Abdollahi, M. (2022). Microfluidically fabricated fibers containing pancreatic islets and mesenchymal stromal cells improve longevity and sustained normoglycemia in diabetic rats. Biofabrication, 15(1). https://doi.org/10.1088/1758-5090/ac9d04

MLA:

Navaei-Nigjeh, Mona, et al. "Microfluidically fabricated fibers containing pancreatic islets and mesenchymal stromal cells improve longevity and sustained normoglycemia in diabetic rats." Biofabrication 15.1 (2022).

BibTeX: Download