Recombinant Spider Silk Bioinks for Continuous Protein Release by Encapsulated Producer Cells

Trossmann VT, Heltmann-Meyer S, Amouei H, Wajant H, Horch RE, Steiner D, Scheibel T (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Biomacromolecules American Chemical Society

Abstract

Targeted therapies using biopharmaceuticals are of growing clinical importance in disease treatment. Currently, there are several limitations of protein-based therapeutics (biologicals), including suboptimal biodistribution, lack of stability, and systemic side effects. A promising approach to overcoming these limitations could be a therapeutic cell-loaded 3D construct consisting of a suitable matrix component that harbors producer cells continuously secreting the biological of interest. Here, the recombinant spider silk proteins eADF4(C16), eADF4(C16)-RGD, and eADF4(C16)-RGE have been processed together with HEK293 producer cells stably secreting the highly traceable reporter biological TNFR2-Fc-GpL, a fusion protein consisting of the extracellular domain of TNFR2, the Fc domain of human IgG1, and the luciferase of Gaussia princeps as a reporter domain. eADF4(C16) and eADF4(C16)-RGD hydrogels provide structural and mechanical support, promote HEK293 cell growth, and allow fusion protein production by the latter. Bioink-captured HEK293 producer cells continuously release functional TNFR2-Fc-GpL over 14 days. Thus, the combination of biocompatible, printable spider silk bioinks with drug-producing cells is promising for generating implantable 3D constructs for continuous targeted therapy.

Authors with CRIS profile

Stefanie Heltmann-Meyer Department of Plastic and Hand Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Dominik Steiner Department of Plastic and Hand Surgery

Involved external institutions

Universität Bayreuth

Germany (DE) Universitätsklinikum Würzburg

Germany (DE)

How to cite

APA:

Trossmann, V.T., Heltmann-Meyer, S., Amouei, H., Wajant, H., Horch, R.E., Steiner, D., & Scheibel, T. (2022). Recombinant Spider Silk Bioinks for Continuous Protein Release by Encapsulated Producer Cells. Biomacromolecules. https://doi.org/10.1021/acs.biomac.2c00971

MLA:

Trossmann, Vanessa T., et al. "Recombinant Spider Silk Bioinks for Continuous Protein Release by Encapsulated Producer Cells." Biomacromolecules (2022).

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