Sensitivity of multifrequency magnetic resonance elastography and diffusion-weighted imaging to cellular and stromal integrity of liver tissue
De Schellenberger AA, Tzschaetzsch H, Polchlopek B, Bertalan G, Schrank F, Garczynska K, Janmey PA, Braun J, Sack I (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 88
Pages Range: 201-208
DOI: 10.1016/j.jbiomech.2019.03.037
Abstract
Microscopic structural alterations of liver tissue induced by freeze-thaw cycles give rise to palpable property changes. However, the underlying damage to tissue architecture is difficult to quantify histologically, and published data on macroscopic changes in biophysical properties are sparse. To better understand the influence of hepatic cells and stroma on global biophysical parameters, we studied rat liver specimens freshly taken (within 30 min after death)and treated by freeze-thaw cycles overnight at either −20 °C or –80 °C using diffusion-weighted imaging (DWI)and multifrequency magnetic resonance elastography (MRE)performed at 0.5 T in a tabletop MRE scanner. Tissue structure was analyzed histologically and rheologic data were analyzed using fractional order derivatives conceptualized by a called spring-pot component that interpolates between pure elastic and viscous responses. Overnight freezing and thawing induced membrane disruptions and cell detachment in the space of Disse, resulting in a markedly lower shear modulus μ and apparent diffusion coefficient (ADC)(μ[−20 °C]= 1.23 ± 0.73 kPa, μ[−80 °C]= 0.66 ± 0.75 kPa; ADC[–20 °C]= 0.649 ± 0.028 μm 2 /s, ADC[−80 °C]= 0.626 ± 0.025 μm 2 /s)compared to normal tissue (μ = 9.92 ± 3.30 kPa, ADC = 0.770 ± 0.023 μm 2 /s, all p < 0.001). Furthermore, we analyzed the springpot-powerlaw coefficient and observed a reduction in −20 °C specimens (0.22 ± 0.14)compared to native tissue (0.40 ± 0.10, p = 0.033)and −80 °C specimens (0.54 ± 0.22, p = 0.002), that correlated with histological observations of sinusoidal dilation and collagen distortion within the space of Disse. Overall, the results suggest that shear modulus and water diffusion in liver tissue markedly decrease due to cell membrane degradation and cell detachment while viscosity-related properties appear to be more sensitive to distorted stromal and microvascular architecture.
Involved external institutions
Charité - Universitätsmedizin Berlin
Germany (DE)
University of Pennsylvania
United States (USA) (US)
Germany (DE)
University of Pennsylvania
United States (USA) (US)
How to cite
APA:
De Schellenberger, A.A., Tzschaetzsch, H., Polchlopek, B., Bertalan, G., Schrank, F., Garczynska, K.,... Sack, I. (2019). Sensitivity of multifrequency magnetic resonance elastography and diffusion-weighted imaging to cellular and stromal integrity of liver tissue. Journal of Biomechanics, 88, 201-208. https://doi.org/10.1016/j.jbiomech.2019.03.037
MLA:
De Schellenberger, Angela Ariza, et al. "Sensitivity of multifrequency magnetic resonance elastography and diffusion-weighted imaging to cellular and stromal integrity of liver tissue." Journal of Biomechanics 88 (2019): 201-208.
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