Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions
Kashkanova AD, Blessing M, Gemeinhardt A, Soulat D, Sandoghdar V (2022)
Publication Type: Journal article
Publication year: 2022
Journal
DOI: 10.1038/s41592-022-01460-z
Abstract
Characterization of the size and material properties of particles in liquid suspensions is in very high demand, for example, in the analysis of colloidal samples or of bodily fluids such as urine or blood plasma. However, existing methods are limited in their ability to decipher the constituents of realistic samples. Here we introduce iNTA as a new method that combines interferometric detection of scattering with nanoparticle tracking analysis to reach unprecedented sensitivity and precision in determining the size and refractive index distributions of nanoparticles in suspensions. After benchmarking iNTA with samples of colloidal gold, we present its remarkable ability to resolve the constituents of various multicomponent and polydisperse samples of known origin. Furthermore, we showcase the method by elucidating the refractive index and size distributions of extracellular vesicles from Leishmania parasites and human urine. The current performance of iNTA already enables advances in several important applications, but we also discuss possible improvements.
Authors with CRIS profile
Didier Soulat
Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene
Vahid Sandoghdar
Involved external institutions
Germany (DE)How to cite
APA:
Kashkanova, A.D., Blessing, M., Gemeinhardt, A., Soulat, D., & Sandoghdar, V. (2022). Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions. Nature Methods. https://doi.org/10.1038/s41592-022-01460-z
MLA:
Kashkanova, Anna D., et al. "Precision size and refractive index analysis of weakly scattering nanoparticles in polydispersions." Nature Methods (2022).
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