Centrosome-dependent microtubule modifications set the conditions for axon formation

Meka DP, Kobler O, Hong S, Friedrich CM, Wuesthoff S, Henis M, Schwanke B, Krisp C, Schmuelling N, Rueter R, Ruecker T, Betleja E, Cheng T, Mahjoub MR, Soba P, Schlüter H, Fornasiero EF, Calderon de Anda F (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 39

Article Number: 110686

Journal Issue: 3

DOI: 10.1016/j.celrep.2022.110686

Abstract

Microtubule (MT) modifications are critical during axon development, with stable MTs populating the axon. How these modifications are spatially coordinated is unclear. Here, via high-resolution microscopy, we show that early developing neurons have fewer somatic acetylated MTs restricted near the centrosome. At later stages, however, acetylated MTs spread out in soma and concentrate in growing axon. Live imaging in early plated neurons of the MT plus-end protein, EB3, show increased displacement and growth rate near the MTOC, suggesting local differences that might support axon selection. Moreover, F-actin disruption in early developing neurons, which show fewer somatic acetylated MTs, does not induce multiple axons, unlike later stages. Overexpression of centrosomal protein 120 (Cep120), which promotes MT acetylation/stabilization, induces multiple axons, while its knockdown downregulates proteins modulating MT dynamics and stability, hampering axon formation. Collectively, we show how centrosome-dependent MT modifications contribute to axon formation.

Authors with CRIS profile

Peter Soba

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Washington University US United States (USA) (US) Leibniz-Institut für Neurobiologie (LIN) DE Germany (DE) Universitätsklinikum Göttingen DE Germany (DE)

How to cite

APA:

Meka, D.P., Kobler, O., Hong, S., Friedrich, C.M., Wuesthoff, S., Henis, M.,... Calderon de Anda, F. (2022). Centrosome-dependent microtubule modifications set the conditions for axon formation. Cell Reports, 39(3). https://doi.org/10.1016/j.celrep.2022.110686

MLA:

Meka, Durga Praveen, et al. "Centrosome-dependent microtubule modifications set the conditions for axon formation." Cell Reports 39.3 (2022).

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