Hydrogel microvalves as control elements for parallelized enzymatic cascade reactions in microfluidics
Obst F, Beck A, Bishayee C, Mehner PJ, Richter A, Voit B, Appelhans D (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 11
Article Number: 167
Journal Issue: 2
DOI: 10.3390/mi11020167
Abstract
Compartmentalized microfluidic devices with immobilized catalysts are a valuable tool for overcoming the incompatibility challenge in (bio) catalytic cascade reactions and high-throughput screening of multiple reaction parameters. To achieve flow control in microfluidics, stimuli-responsive hydrogel microvalves were previously introduced. However, an application of this valve concept for the control of multistep reactions was not yet shown. To fill this gap, we show the integration of thermoresponsive poly(N-isopropylacrylamide) (PNiPAAm) microvalves (diameter: 500 and 600 μm) into PDMS-on-glass microfluidic devices for the control of parallelized enzyme-catalyzed cascade reactions. As a proof-of-principle, the biocatalysts glucose oxidase (GOx), horseradish peroxidase (HRP) and myoglobin (Myo) were immobilized in photopatterned hydrogel dot arrays (diameter of the dots: 350 μm, amount of enzymes: 0.13-2.3 μg) within three compartments of the device. Switching of the microvalves was achieved within 4 to 6 s and thereby the fluid pathway of the enzyme substrate solution (5 mmol/L) in the device was determined. Consequently, either the enzyme cascade reaction GOx-HRP or GOx-Myo was performed and continuously quantified by ultraviolet-visible (UV-Vis) spectroscopy. The functionality of the microvalves was shown in four hourly switching cycles and visualized by the path-dependent substrate conversion.
Involved external institutions
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
Technische Universität Dresden
Germany (DE)
Germany (DE)
Technische Universität Dresden
Germany (DE)
How to cite
APA:
Obst, F., Beck, A., Bishayee, C., Mehner, P.J., Richter, A., Voit, B., & Appelhans, D. (2020). Hydrogel microvalves as control elements for parallelized enzymatic cascade reactions in microfluidics. Micromachines, 11(2). https://doi.org/10.3390/mi11020167
MLA:
Obst, Franziska, et al. "Hydrogel microvalves as control elements for parallelized enzymatic cascade reactions in microfluidics." Micromachines 11.2 (2020).
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