Contrast-Enhanced µCT for Visualizing and Evaluating Murine Intestinal Inflammation

Journal article


Publication Details

Author(s): Jung D, Heiß R, Kramer V, Thoma OM, Regensburger A, Rascher W, Uder M, Neurath M, Knieling F, Waldner M
Journal: Theranostics
Publication year: 2018
Volume: 8
Journal issue: 22
Pages range: 6357-6366
ISSN: 1838-7640


Abstract

Rationale: To develop a simple and fast protocol for the assessment of acute and chronic experimental intestinal inflammation using contrast-enhanced µCT. Methods: For the imaging studies, an acute 2% and 3% dextran sodium sulfate (n = 15, female, 8-12 weeks) and a chronic adoptive transfer colitis model (n = 10, female, 8-9 weeks) were established over 9 days or 6 weeks, respectively. Throughout the experiments, longitudinal measurement of murine intestinal wall thickness and time dependent perfusion was performed on a small animal µCT system (90 kV, 160 μA, FOV: 60 mm, scan time: 17 s, image size: 512x512, layer thickness: 118 µm) between 0.5 and 30 min after intravenous bolus injection of an iodine contrast agent. Weight development, small animal endoscopy, and histological ex vivo analysis were compared to contrast-enhanced µCT imaging findings. Results: Murine intestinal wall thickness was significantly increased in inflamed colons of acute colitis at day 9 in comparison to pre-inflamed state. Perfusion analysis revealed a late contrast enhancement in acute inflamed colons and the renal medulla at day 9 compared to control mice. An increasing intestinal wall thickness was monitored 3, 5 and 6 weeks after on-set of chronic colitis in comparison to controls. A good correlation with endoscopic (r = 0.75, p < 0.0001) and histologic degree of inflammation (r = 0.83, p = 0.04) was found. Conclusion: Contrast-enhanced µCT is a simple and fast method to assess acute intestinal inflammation and to monitor disease progression in experimental models of chronic colitis. According to our findings, one single contrast-enhanced µCT-scan is a valid non-invasive modality to quantify the degree of inflammation in the entire digestive tract in murine inflammatory models.


FAU Authors / FAU Editors

Heiß, Rafael Dr. med.
Professur für Innere Medizin (Hochdruckforschung)
Knieling, Ferdinand Dr. med.
Kinder- und Jugendklinik
Neurath, Markus Prof. Dr.
Lehrstuhl für Innere Medizin I
Rascher, Wolfgang Prof. Dr. med.
Kinder- und Jugendklinik
Regensburger, Adrian Dr. med.
Kinder- und Jugendklinik
Uder, Michael Prof. Dr.
Lehrstuhl für Diagnostische Radiologie
Waldner, Maximilian Prof. Dr.
Lehrstuhl für Innere Medizin I


How to cite

APA:
Jung, D., Heiß, R., Kramer, V., Thoma, O.-M., Regensburger, A., Rascher, W.,... Waldner, M. (2018). Contrast-Enhanced µCT for Visualizing and Evaluating Murine Intestinal Inflammation. Theranostics, 8(22), 6357-6366. https://dx.doi.org/10.7150/thno.26013

MLA:
Jung, Dennis, et al. "Contrast-Enhanced µCT for Visualizing and Evaluating Murine Intestinal Inflammation." Theranostics 8.22 (2018): 6357-6366.

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Last updated on 2019-18-07 at 07:12