Absolute quantification of two antimicrobial peptides alpha(S2)-casein(182-207) and alpha(S2)-casein(151-181) in bovine milk by UHPLC-ESI-MS/MS in sMRM mode

Journal article


Publication Details

Author(s): Liu Y, Pischetsrieder M
Journal: Food Chemistry
Publisher: ELSEVIER SCI LTD
Publication year: 2018
Volume: 261
Pages range: 15-20
ISSN: 0308-8146


Abstract

To evaluate if the recently identified antimicrobial milk peptides alpha(S2)-casein(182-207) and alpha(S2)-casein(151-181) can be of physiological or technological relevance, their presence in commercial milk was investigated. Thus, a UHPLC-ESI-MS/MS scheduled multiple reaction monitoring method was developed for quantification using stable isotope-labeled peptides. The developed protocol gave very good linear response (R-2 > 0.99) for both peptides, recovery > 95% and coefficients of variations of 5.51% and 4.79%. The limits of detection (0.3 nM alpha(S2)-casein(182-207) and 0.5 nM alpha(S2)-casein(151-181)) were low enough to detect both peptides without further enrichment. The highest peptide concentration was recorded in pasteurized milk (1.36 mu M alpha(S2)-casein(182-207) and 0.20 mu M alpha(S2)-casein(151-181)). The concentration of alpha(S2)-casein(182-207) was in a range that was previously reported to act bacteriostatic against Bacillus subtilis. Industrial milk processing led to a significant loss of both peptides resulting in a mean concentration of 0.47 mu M alpha(S2)-casein(182-207) and 0.098 mu M alpha(S2)-casein(151-181) in ultrahigh-temperature milk.


FAU Authors / FAU Editors

Liu, Yufang
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)
Pischetsrieder, Monika Prof. Dr.
Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)


Additional Organisation
Emil-Fischer-Zentrum (Emil Fischer Center)


How to cite

APA:
Liu, Y., & Pischetsrieder, M. (2018). Absolute quantification of two antimicrobial peptides alpha(S2)-casein(182-207) and alpha(S2)-casein(151-181) in bovine milk by UHPLC-ESI-MS/MS in sMRM mode. Food Chemistry, 261, 15-20. https://dx.doi.org/10.1016/j.foodchem.2018.04.004

MLA:
Liu, Yufang, and Monika Pischetsrieder. "Absolute quantification of two antimicrobial peptides alpha(S2)-casein(182-207) and alpha(S2)-casein(151-181) in bovine milk by UHPLC-ESI-MS/MS in sMRM mode." Food Chemistry 261 (2018): 15-20.

BibTeX: 

Last updated on 2019-04-01 at 14:10