Membrane-proximal tryptophans of synaptobrevin II stabilize priming of secretory vesicles.
Borisovska M, Schwarz YN, Dhara M, Yarzagaray A, Hugo S, Narzi D, Siu SWI, Kesavan J, Mohrmann R, Böckmann R, Bruns D (2012)
Publication Language: English
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2012
Journal
Publisher: Society for Neuroscience
Book Volume: 32
Pages Range: 15983-97
Volume: 32
Issue: 45
Journal Issue: 45
DOI: 10.1523/JNEUROSCI.6282-11.2012
Abstract
Trans-soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complexes formed between the SNARE motifs of synaptobrevin II, SNAP-25, and syntaxin play an essential role in Ca(2+)-regulated exocytosis. Apart from the well studied interactions of the SNARE domains, little is known about the functional relevance of other evolutionarily conserved structures in the SNARE proteins. Here, we show that substitution of two highly conserved tryptophan residues within the juxtamembrane domain (JMD) of the vesicular SNARE Synaptobrevin II (SybII) profoundly impairs priming of granules in mouse chromaffin cells without altering catecholamine release from single vesicles. Using molecular dynamic simulations of membrane-embedded SybII, we show that Trp residues of the JMD influence the electrostatic surface potential by controlling the position of neighboring lysine and arginine residues at the membrane-water interface. Our observations indicate a decisive role of the tryptophan moiety of SybII in keeping the vesicles in the release-ready state and support a model wherein tryptophan-mediated protein-lipid interactions assist in bridging the apposing membranes before fusion.
Authors with CRIS profile
Daniele Narzi
Professur für Computational Biology
Shirley Weng In Siu
Professur für Computational Biology
Rainer Böckmann
Professur für Computational Biology
Involved external institutions
Germany (DE)How to cite
APA:
Borisovska, M., Schwarz, Y.N., Dhara, M., Yarzagaray, A., Hugo, S., Narzi, D.,... Bruns, D. (2012). Membrane-proximal tryptophans of synaptobrevin II stabilize priming of secretory vesicles. The Journal of Neuroscience, 32(45), 15983-97. https://doi.org/10.1523/JNEUROSCI.6282-11.2012
MLA:
Borisovska, Maria, et al. "Membrane-proximal tryptophans of synaptobrevin II stabilize priming of secretory vesicles." The Journal of Neuroscience 32.45 (2012): 15983-97.
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