Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Lelental N, Brandner S, Kofanova O, Blennow K, Zetterberg H, Andreasson U, Engelborghs S, Mroczko B, Gabryelewicz T, Teunissen C, Mollenhauer B, Parnetti L, Chiasserini D, Molinuevo JL, Perret-Liaudet A, Verbeek MM, Andreasen N, Brosseron F, Bahl JMC, Herukka SK, Hausner L, Froelich L, Labonte A, Poirier J, Miller AM, Zilka N, Kovacech B, Urbani A, Suardi S, Oliveira C, Baldeiras I, Dubois B, Rot U, Lehmann S, Skinningsrud A, Betsou F, Wiltfang J, Gkatzima O, Winblad B, Buchfelder M, Kornhuber J, Lewczuk P
Zeitschrift: Journal of Alzheimers Disease
Jahr der Veröffentlichung: 2016
Band: 52
Heftnummer: 1
Seitenbereich: 51-64
ISSN: 1387-2877


Assay-vendor independent quality control (QC) samples for neurochemical dementia diagnostics (NDD) biomarkers are so far commercially unavailable. This requires that NDD laboratories prepare their own QC samples, for example by pooling leftover cerebrospinal fluid (CSF) samples.To prepare and test alternative matrices for QC samples that could facilitate intra- and inter-laboratory QC of the NDD biomarkers.Three matrices were validated in this study: (A) human pooled CSF, (B) A? peptides spiked into human prediluted plasma, and (C) A? peptides spiked into solution of bovine serum albumin in phosphate-buffered saline. All matrices were tested also after supplementation with an antibacterial agent (sodium azide). We analyzed short- and long-term stability of the biomarkers with ELISA and chemiluminescence (Fujirebio Europe, MSD, IBL International), and performed an inter-laboratory variability study.NDD biomarkers turned out to be stable in almost all samples stored at the tested conditions for up to 14 days as well as in samples stored deep-frozen (at - 80°C) for up to one year. Sodium azide did not influence biomarker stability. Inter-center variability of the samples sent at room temperature (pooled CSF, freeze-dried CSF, and four artificial matrices) was comparable to the results obtained on deep-frozen samples in other large-scale projects.Our results suggest that it is possible to replace self-made, CSF-based QC samples with large-scale volumes of QC materials prepared with artificial peptides and matrices. This would greatly facilitate intra- and inter-laboratory QC schedules for NDD measurements.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Brandner, Sebastian PD Dr.
Neurochirurgische Klinik
Buchfelder, Michael Prof. Dr.
Lehrstuhl für Neurochirurgie
Kornhuber, Johannes Prof. Dr. med.
Lehrstuhl für Psychiatrie und Psychotherapie
Ermann, Natalia
Medizinische Fakultät
Lewczuk, Piotr Prof. Dr.
Psychiatrische und Psychotherapeutische Klinik

Einrichtungen weiterer Autorinnen und Autoren

Akershus University Hospital / Akershus universitetssykehus (Ahus)
Aristotle University of Thessaloniki
Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)
Douglas Mental Health University Institute
Foundation of the Carlo Besta Neurological Institute (IRCCS)
Foundation Saint Lucia
Georg-August-Universität Göttingen
Hospital Clínic de Barcelona
Integrated Biobank of Luxembourg: IBBL
Karolinska Institute
Karolinska University Hospital / Karolinska Universitetssjukhuset
Paracelsus-Elena-Klinik Kassel
Polska Akademia Nauk (PAN) / Polish Academy of Sciences
Radboud University Nijmegen
Ruprecht-Karls-Universität Heidelberg
Slovak Academy of Sciences (SAS) / Slovenská akadémia vied (SAV)
Statens Serum Institut
Trinity College Dublin
Universidade de Coimbra
Università degli Studi di Perugia
Universitätsklinikum Heidelberg
Université Claude Bernard Lyon 1 (UCB)
University hospital Montpellier CHRU
University of Antwerp / Universiteit Antwerpen
University of Białystok
University of Eastern Finland
University of Gothenburg / Göteborgs universitet
University of Paris 6 - Pierre et Marie Curie / Université Paris VI Pierre et Marie Curie (UPMC)
Vrije Universiteit Amsterdam (VU) / University Amsterdam


Lelental, N., Brandner, S., Kofanova, O., Blennow, K., Zetterberg, H., Andreasson, U.,... Lewczuk, P. (2016). Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics. Journal of Alzheimers Disease, 52(1), 51-64.

Lelental, Natalia, et al. "Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics." Journal of Alzheimers Disease 52.1 (2016): 51-64.


Zuletzt aktualisiert 2018-09-10 um 02:18