Closely related, yet unique: Distinct homo- and heterodimerization patterns of G protein coupled chemokine receptors and their fine-tuning by cholesterol.

Beitrag in einer Fachzeitschrift
(Originalarbeit)


Details zur Publikation

Autor(en): Gahbauer S, Pluhackova K, Böckmann R
Zeitschrift: PLoS Computational Biology
Jahr der Veröffentlichung: 2018
Band: 14
Heftnummer: 3
Seitenbereich: e1006062
ISSN: 1553-734X
eISSN: 1553-7358
Sprache: Englisch


Abstract


Chemokine receptors, a subclass of G protein coupled receptors (GPCRs), play essential roles in the human immune system, they are involved in cancer metastasis as well as in HIV-infection. A plethora of studies show that homo- and heterodimers or even higher order oligomers of the chemokine receptors CXCR4, CCR5, and CCR2 modulate receptor function. In addition, membrane cholesterol affects chemokine receptor activity. However, structural information about homo- and heterodimers formed by chemokine receptors and their interplay with cholesterol is limited. Here, we report homo- and heterodimer configurations of the chemokine receptors CXCR4, CCR5, and CCR2 at atomistic detail, as obtained from thousands of molecular dynamics simulations. The observed homodimerization patterns were similar for the closely related CC chemokine receptors, yet they differed significantly between the CC receptors and CXCR4. Despite their high sequence identity, cholesterol modulated the CC homodimer interfaces in a subtype-specific manner. Chemokine receptor heterodimers display distinct dimerization patterns for CXCR4/CCR5 and CXCR4/CCR2. Furthermore, associations between CXCR4 and CCR5 reveal an increased cholesterol-sensitivity as compared to CXCR4/CCR2 heterodimerization patterns. This work provides a first comprehensive structural overview over the complex interaction network between chemokine receptors and indicates how heterodimerization and the interaction with the membrane environment diversifies the function of closely related GPCRs.


FAU-Autoren / FAU-Herausgeber

Böckmann, Rainer Prof. Dr.
Professur für Computational Biology
Gahbauer, Stefan Dr.
Professur für Computational Biology
Pluhackova, Kristyna
Professur für Computational Biology


Zitierweisen

APA:
Gahbauer, S., Pluhackova, K., & Böckmann, R. (2018). Closely related, yet unique: Distinct homo- and heterodimerization patterns of G protein coupled chemokine receptors and their fine-tuning by cholesterol. PLoS Computational Biology, 14(3), e1006062. https://dx.doi.org/10.1371/journal.pcbi.1006062

MLA:
Gahbauer, Stefan, Kristyna Pluhackova, and Rainer Böckmann. "Closely related, yet unique: Distinct homo- and heterodimerization patterns of G protein coupled chemokine receptors and their fine-tuning by cholesterol." PLoS Computational Biology 14.3 (2018): e1006062.

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Zuletzt aktualisiert 2019-03-06 um 08:53