Agonist-dependent modulation of cell surface expression of the cold receptor TRPM8

Beitrag in einer Fachzeitschrift

Details zur Publikation

Autorinnen und Autoren: Toro CA, Eger S, Veliz L, Sotelo-Hitschfeld P, Cabezas D, Castro MA, Zimmermann K, Brauchi S
Zeitschrift: The Journal of Neuroscience
Jahr der Veröffentlichung: 2015
Band: 35
Heftnummer: 2
Seitenbereich: 571-82
ISSN: 1529-2401


The spatial and temporal distribution of receptors constitutes an important mechanism for controlling the magnitude of cellular responses. Several members of the transient receptor potential (TRP) ion channel family can regulate their function by modulating their expression at the plasma membrane (PM) through rapid vesicular translocation and fusion. The mechanisms underlying this regulation are not completely understood, and the contribution of vesicular trafficking to physiological function is unknown. TRPM8 receptors are expressed in mammalian peripheral sensory neurons and are essential for the detection of cold temperatures. Previously, we showed that TRPM8-containing vesicles are segregated into three main pools, immobile at the PM, simple diffusive and corralled-hopping. Here, we show that channel expression at the PM is modulated by TRPM8 agonists in F11 and HEK293T cells. Our results support a model in which the activation of TRPM8 channels, located at the PM, induces a short-lived recruitment of a TRPM8-containing vesicular pool to the cell surface causing a transitory increase in the number of functional channels, affecting intrinsic properties of cold receptor responses. We further demonstrate the requirement of intact vesicular trafficking to support sustained cold responses in the skin of mice.

FAU-Autorinnen und Autoren / FAU-Herausgeberinnen und Herausgeber

Eger, Stephanie
Medizinische Fakultät
Zimmermann, Katharina Prof. Dr. med.
Heisenbergprofessur für Experimentelle Schmerzforschung

Einrichtungen weiterer Autorinnen und Autoren

Universidad Austral de Chile


Toro, C.A., Eger, S., Veliz, L., Sotelo-Hitschfeld, P., Cabezas, D., Castro, M.A.,... Brauchi, S. (2015). Agonist-dependent modulation of cell surface expression of the cold receptor TRPM8. The Journal of Neuroscience, 35(2), 571-82.

Toro, Carlos A., et al. "Agonist-dependent modulation of cell surface expression of the cold receptor TRPM8." The Journal of Neuroscience 35.2 (2015): 571-82.


Zuletzt aktualisiert 2018-06-10 um 02:44