Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop

Paolini L, Tassoni S, Al-Jamal KT, Banquy X, Beke-Somfai T, Bergese P, Boros FA, Bottini M, Boyd B, Busatto S, Bussolati B, Canepa E, Castagnola V, Clayton A, Dawson K, El-Andaloussi S, Försönits A, Fortunato O, Frigerio R, Gabrielli M, Grange C, Guescini M, Hayashi Y, Hemmingsen JK, Kim S, Kinga I, Kyykallio H, Lenassi M, Lima LG, Mahoney Mỹ, Vandenbroucke CM, Möller A, Musicò A, Nelissen I, Nogueira LFB, Nöhammer C, Parisse P, Ramassamy C, Roballo KCS, Salvati A, Sancho-Albero M, Shelke G, Shirmast P, Sonallya T, Szigyártó IC, van Eijndhoven MA, Viitala T, Wang S, Yan Y, Zendrini A, Buzás E, Radeghieri A (2026)

Publication Type: Journal article

Publication year: 2026

Journal

Book Volume: 5

Article Number: e70172

Journal Issue: 8

DOI: 10.1002/jex2.70172

Abstract

The concept of the “biomolecular corona” (BC) was first introduced to describe the spontaneous absorption of molecules, mainly proteins, onto the surface of synthetic nanoparticles upon exposure to biological fluids. More recently, the same term has been used to describe biomolecules that dynamically interact with the surface of extracellular vesicles (EVs) during their biogenesis, secretion, or upon exposure to intracellular or extracellular milieus, including biological fluids. The EV biomolecular corona is a key determinant of EV identity, shaping their cellular uptake, biodistribution, and functional outcomes in both physiological and pathological contexts. EVs, in contrast to synthetic nanoparticles, introduce distinct challenges and perspectives due to their biological origin, innate heterogeneity, and complex combination of physicochemical and biological properties. To address this evolving topic, the International Society for Extracellular Vesicles (ISEV) organized a dedicated workshop in Brescia, Italy, on 27th to 28th February, 2025. The event gathered selected participants from academia and industry, with expertise spanning synthetic nanomaterials, EVs, biophysics, and translational nanomedicine. The program included keynote lectures, oral presentations, posters, and a series of interactive roundtables structured around fundamentals of the biomolecular corona, EV corona biogenesis and composition, analytical and separation tools and clinical and diagnostic potential applications. This report summarizes the key scientific insights, methodological challenges, conceptual debates, and future perspectives that emerged from the workshop.

Authors with CRIS profile

Involved external institutions

Università degli Studi di Roma 'Tor Vergata' Italy (IT) Monash University Australia (AU) University of Groningen / Rijksuniversiteit Groningen Netherlands (NL) University of Turin / Università degli Studi di Torino (UNITO) Italy (IT) University College Dublin (UCD) Ireland (IE) Fondazione Pisana per la Scienza Onlus Italy (IT) Karolinska Institute Sweden (SE) Semmelweis University / Semmelweis Egyetem Hungary (HU) Fondazione IRCCS: Istituto Nazionale dei Tumori Italy (IT) Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich Switzerland (CH) Nottingham Trent University United Kingdom (GB) University of Urbino "Carlo Bo" / Università degli Studi di Urbino "Carlo Bo" (UNIURB) Italy (IT) Aarhus University Denmark (DK) Korea University / 고려대학교 Korea, Republic of (KR) HUN-REN Research Centre for Natural Sciences / HUN-REN Természettudományi Kutatóközpont Hungary (HU) University of Eastern Finland Finland (FI) University of Ljubljana (UL) / Univerza v Ljubljani Slovenia (SI) The Chinese University of Hong Kong (CUHK) China (CN) Thomas Jefferson University Hospitals United States (USA) (US) Consiglio Nazionale delle Ricerche (CNR) / National Research Council of Italy Italy (IT) Flemish Institute for Technological Research / Vlaamse Instelling voor Technologisch Onderzoek (VITO) Belgium (BE) University of São Paulo / Universidade de São Paulo (USP) Brazil (BR) AIT Austrian Institute of Technology Austria (AT) IOM - Istituto Officina dei Materiali Italy (IT) US National Institutes of Health (NIH) United States (USA) (US) Griffith University Australia (AU) Vrije Universiteit Amsterdam (VU) / University Amsterdam Netherlands (NL) Cardiff University United Kingdom (GB) Institut National de la Recherche Scientifique (INRS) de l'Université du Québec Canada (CA) Edward Via College of Osteopathic Medicine United States (USA) (US) Universidad de Zaragoza Spain (ES) Università degli Studi di Brescia Italy (IT) Center for Colloid and Surface Science (CSGI) Italy (IT) Université de Montréal Canada (CA) Helsingin yliopisto / University of Helsinki Finland (FI) Huazhong University of Science & Technology China (CN)

How to cite

APA:

Paolini, L., Tassoni, S., Al-Jamal, K.T., Banquy, X., Beke-Somfai, T., Bergese, P.,... Radeghieri, A. (2026). Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop. Journal of Extracellular Biology, 5(8). https://doi.org/10.1002/jex2.70172

MLA:

Paolini, Lucia, et al. "Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop." Journal of Extracellular Biology 5.8 (2026).

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