Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles

Boros FA, Arnold P, Regensburger M, Zunke F (2026)

Publication Type: Journal article

Publication year: 2026

Journal

STAR Protocols Cell Press

Book Volume: 7

Article Number: 104735

Journal Issue: 3

DOI: 10.1016/j.xpro.2026.104735

Abstract

Extracellular vesicles (EVs) are gaining increasing attention among studies focusing on biomarker research, elucidating disease mechanisms, and exploring therapeutic approaches. Here, we present a method for size-exclusion chromatography (SEC)-based enrichment of human plasma EVs, and describe steps for assessing the quality of obtained EV samples. For characterizing the EVs, we provide detailed procedures for western blot, transmission electron microscopy, and nanoparticle tracking analysis techniques.

Authors with CRIS profile

Fanni Annamaria Lazarov-Boros Department of Molecular Neurology Philipp Arnold Lehrstuhl für Funktionelle und Klinische Anatomie Martin Regensburger Department of Molecular Neurology Friederike Zunke Department of Molecular Neurology

How to cite

APA:

Boros, F.A., Arnold, P., Regensburger, M., & Zunke, F. (2026). Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles. STAR Protocols, 7(3). https://doi.org/10.1016/j.xpro.2026.104735

MLA:

Boros, Fanni Annamaria, et al. "Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles." STAR Protocols 7.3 (2026).

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