Bitter but beneficial: Hop-based feed additives for fighting antimicrobial resistance in poultry

Kober L, Strauch SM, Graßl V, Fröhlich Tonet M, Hirschmann N, Fischer D, Erzinger GS, Castiglione K (2026)

Publication Type: Journal article

Publication year: 2026

Journal

Poultry Science Poultry Science Association

Book Volume: 105

Article Number: 107376

Journal Issue: 10

DOI: 10.1016/j.psj.2026.107376

Abstract

Antimicrobial resistance (AMR) is an increasing challenge in the global production of poultry, where antibiotics have traditionally been used to promote growth. Phytogenic feed additives (PFAs), particularly hop-based ones, could be a sustainable alternative due to their antimicrobial properties. The present study investigated two hop extracts from two sources, which are Type 90 hop pellets derived from fresh hops and residue of same hop pellets that had previously undergone CO2 extraction, using a cross-model analysis combining in vitro (viability assay/broth dilution assay), ex ovo (shell-less hen's egg test) and in vivo tests. Both types of extracts exhibited antimicrobial activity in vitro and high biocompatibility in the ex ovo model. Notably, the biocompatibility was improved for ex ovo setup, compared to in vitro cytotoxicity, providing important insights regarding their potential application as PFAs. For in vivo studies, hop extracts were microencapsulated as solid lipid microspheres to increase their stability and bioavailability. Feeding animals microencapsulated hop extracts or xanthohumol-rich residue from CO2 extraction led to a significant reduction specifically of Gram-negative intestinal bacteria, with no change in animal performance. Overall, two promising products were identified that could potentially be used as a sustainable alternative to antibiotics in poultry production.

Authors with CRIS profile

Luisa Kober Lehrstuhl für Bioverfahrenstechnik Veronika Graßl Lehrstuhl für Pharmazeutische Technologie und Biopharmazie Nora Hirschmann Lehrstuhl für Bioverfahrenstechnik Dagmar Fischer Lehrstuhl für Pharmazeutische Technologie und Biopharmazie Kathrin Castiglione Lehrstuhl für Bioverfahrenstechnik

Involved external institutions

Universidade da Região de Joinville (UNIVILLE) BR Brazil (BR)

How to cite

APA:

Kober, L., Strauch, S.M., Graßl, V., Fröhlich Tonet, M., Hirschmann, N., Fischer, D.,... Castiglione, K. (2026). Bitter but beneficial: Hop-based feed additives for fighting antimicrobial resistance in poultry. Poultry Science, 105(10). https://doi.org/10.1016/j.psj.2026.107376

MLA:

Kober, Luisa, et al. "Bitter but beneficial: Hop-based feed additives for fighting antimicrobial resistance in poultry." Poultry Science 105.10 (2026).

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