Intratumoral distribution of anisokaryosis in canine cutaneous mast cell tumors
Bertram CA, Wilm F, Parlak E, Donovan TA, Janout H, Bolfa P, Dark MJ, Fuchs-Baumgartinger A, Klang A, Klopfleisch R, Richter B, Aubreville M, Winkler SM, Kiupel M, Bartel A (2026)
Publication Type: Journal article
Publication year: 2026
Journal
DOI: 10.1177/03009858261465447
Abstract
Automated measurements of anisokaryosis in canine cutaneous mast cell tumors (ccMCTs) have been shown to be predictive of survival, but questions remain regarding the intratumoral distribution of anisokaryosis. Whole-slide images of 96 ccMCTs were analyzed with a deep learning-based segmentation algorithm to quantify anisokaryosis using the standard deviation (SD) of the nuclear area. In 35/96 cases, >5% of the non-overlapping 256 × 256 µm2 regions were hotspots (SD ≥11.5 µm2). Regions selected by 7 pathologists within these 35 cases matched hotspots in 32% of the instances. Outcome analysis (tumor-related death) based on single tumor regions yielded an area under the curve (AUC) of 0.901 for pathologist-selected hotspots, falling between random region selection (AUC: 0.862) and 90th-percentile targeted selection (AUC: 0.956). Whole-slide analysis of the hotspot proportion predicted survival with an AUC of 0.956, with 20% of hotspots as a prognostically meaningful threshold. Whereas pathologists-selected tumor regions are prognostically meaningful for nuclear morphometry, whole-slide analysis may provide additional prognostic information.
Authors with CRIS profile
Involved external institutions
Veterinärmedizinische Universität Wien (Vetmeduni Vienna) / University of Veterinary Medicine, Vienna
Austria (AT)
Freie Universität Berlin
Germany (DE)
Michigan State University
United States (USA) (US)
Fachhochschule Flensburg
Germany (DE)
Fachhochschule Oberösterreich (FH OÖ) / University of Applied Sciences Upper Austria
Austria (AT)
University of Florida
United States (USA) (US)
Ross University School of Veterinary Medicine
Saint Kitts And Nevis (KN)
The Schwarzman Animal Medical Center (AMC)
United States (USA) (US)
Austria (AT)
Freie Universität Berlin
Germany (DE)
Michigan State University
United States (USA) (US)
Fachhochschule Flensburg
Germany (DE)
Fachhochschule Oberösterreich (FH OÖ) / University of Applied Sciences Upper Austria
Austria (AT)
University of Florida
United States (USA) (US)
Ross University School of Veterinary Medicine
Saint Kitts And Nevis (KN)
The Schwarzman Animal Medical Center (AMC)
United States (USA) (US)
How to cite
APA:
Bertram, C.A., Wilm, F., Parlak, E., Donovan, T.A., Janout, H., Bolfa, P.,... Bartel, A. (2026). Intratumoral distribution of anisokaryosis in canine cutaneous mast cell tumors. Veterinary Pathology. https://doi.org/10.1177/03009858261465447
MLA:
Bertram, Christof A., et al. "Intratumoral distribution of anisokaryosis in canine cutaneous mast cell tumors." Veterinary Pathology (2026).
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