Fibroblast dynamics in colorectal cancer: stability, plasticity, and novel markers

Demmler R, Anchang CG, Yong Y, Ramming A, Rauber S, Schellerer V, Schmid B, Hartmann A, Merkel S, Imkeller K, Naschberger E, Stürzl M (2026)

Publication Type: Journal article

Publication year: 2026

Journal

Oncogene Springer Nature

DOI: 10.1038/s41388-026-03809-6

Abstract

Colorectal cancer (CRC) is one of the most commonly diagnosed and globally spread malignant diseases. Cancer-associated fibroblasts (CAFs) are key architects of the tumor microenvironment, yet their origin, stability, and interconvertibility remain poorly understood. Using transcriptomic profiling of fibroblasts from colorectal cancer (CRC) patients, we identify highly expressed (HEX) markers that define fibroblast subpopulations and uncover mechanisms governing their plasticity. We find that ADH1B marks normal colon-associated fibroblasts (NAFs), which consist of PI16-NAFs and ADAMDEC1-NAFs. ITGA3 delineates the total CAF population, which comprises myofibroblastic CAFs (myCAFs), whose characterizing markers were associated with poor prognosis and proteolytic inflammatory CAFs (piCAFs), characterized by markers not associated with prognosis. An AGT/TGM2-expressing fibroblast subset is present in both healthy and tumor tissues, suggesting alternative trajectories to the classical NAF-to-CAF transition model. While PI16-NAFs, AGT/TGM2-fibroblasts, and myCAFs maintain stable identities in long-term culture, the ADAMDEC1-NAF and piCAF phenotypes are lost in vitro. ITGA3-CAFs demonstrate dynamic plasticity, with TGF-β stably inducing myCAF formation and TNF-α or inhibition of DNA methylation promoting transient piCAF emergence. These findings redefine fibroblast heterogeneity in CRC and reveal a coexisting stable and plastic fibroblast network that may be amenable to modulation and provides a framework for future functional and translational studies. (Figure presented.)

Authors with CRIS profile

Richard Demmler Professur für Molekulare und Experimentelle Chirurgie Charles Gwellem Anchang Department of Medicine 3 – Rheumatology and Immunology Andreas Ramming Department of Medicine 3 – Rheumatology and Immunology Simon Rauber Department of Medicine 3 – Rheumatology and Immunology Vera Schellerer Department of Surgery Benjamin Schmid FAU Optical Imaging Competence Center (FAU OICE) Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Susanne Merkel Department of Surgery Elisabeth Naschberger Medizinische Fakultät Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie

Involved external institutions

Universitätsklinikum Frankfurt am Main (KGU) DE Germany (DE)

How to cite

APA:

Demmler, R., Anchang, C.G., Yong, Y., Ramming, A., Rauber, S., Schellerer, V.,... Stürzl, M. (2026). Fibroblast dynamics in colorectal cancer: stability, plasticity, and novel markers. Oncogene. https://doi.org/10.1038/s41388-026-03809-6

MLA:

Demmler, Richard, et al. "Fibroblast dynamics in colorectal cancer: stability, plasticity, and novel markers." Oncogene (2026).

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