Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103+ cDC1s and CD301b+ cDC2s

Vurnek D, Tchitashvili G, Seichter A, Vuletic T, Heß I, Trapaidze L, Heger L, Tochoedo NR, Lehmann C, Lambrecht BN, Dudziak D, Amon L (2026)

Publication Type: Journal article

Publication year: 2026

Journal

Book Volume: 7

Article Number: 104508

Journal Issue: 2

DOI: 10.1016/j.xpro.2026.104508

Abstract

Ex vivo differentiation platforms have a long-standing history for studying dendritic cell (DC) ontogeny and function. Here, we present a protocol for differentiating bona fide DCs from murine bone marrow. We describe steps for bone marrow preparation, followed by ex vivo differentiation of DC subtypes resembling either lymphoid or mucosal tissue DC phenotypes. We then detail procedures for flow cytometric analysis and sorting strategies that facilitate phenotypic and functional studies of murine DC subtypes and/or their bone marrow progenitors. For complete details on the use and execution of this protocol, please refer to Amon et al. ¹

Authors with CRIS profile

Involved external institutions

Universitätsklinikum Jena Germany (DE) Institut za fiziku / Institute of Physics Croatia (HR) Flanders Institute for Biotechnology / Vlaams Instituut voor Biotechnologie (VIB) Belgium (BE)

How to cite

APA:

Vurnek, D., Tchitashvili, G., Seichter, A., Vuletic, T., Heß, I., Trapaidze, L.,... Amon, L. (2026). Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103+ cDC1s and CD301b+ cDC2s. STAR Protocols, 7(2). https://doi.org/10.1016/j.xpro.2026.104508

MLA:

Vurnek, Damir, et al. "Protocol for mapping murine myeloid bone marrow progenitors and their differentiation into CD103+ cDC1s and CD301b+ cDC2s." STAR Protocols 7.2 (2026).

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