Fast wide-field light sheet electro-optic FLIM
Knight VR, Bode N, Dowlatshahi DP, Kim JG, Mudgett MB, Wakatsuki S, Bowman AJ, Kasevich MA (2026)
Publication Type: Journal article
Publication year: 2026
Journal
Book Volume: 34
Pages Range: 15065-15073
Journal Issue: 8
DOI: 10.1364/OE.581295
Abstract
We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet electro-optic FLIM (LS-EO-FLIM) acquisition with up to 800 µm field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.
Authors with CRIS profile
Involved external institutions
Stanford University
United States (USA) (US)
Stanford National Accelerator Laboratory (SLAC)
United States (USA) (US)
Salk Institute for Biological Studies
United States (USA) (US)
United States (USA) (US)
Stanford National Accelerator Laboratory (SLAC)
United States (USA) (US)
Salk Institute for Biological Studies
United States (USA) (US)
How to cite
APA:
Knight, V.R., Bode, N., Dowlatshahi, D.P., Kim, J.G., Mudgett, M.B., Wakatsuki, S.,... Kasevich, M.A. (2026). Fast wide-field light sheet electro-optic FLIM. Optics Express, 34(8), 15065-15073. https://doi.org/10.1364/OE.581295
MLA:
Knight, V. Rose, et al. "Fast wide-field light sheet electro-optic FLIM." Optics Express 34.8 (2026): 15065-15073.
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