Detection of Pseudomonas aeruginosa in Whole Blood using a portable Magnetic Particle Spectrometer-a feasibility study

Heinen L, Simonis A, Rybniker J, Günther J, Vogel P, Janko C, Alexiou C, Lyer S, Tietze R (2026)

Publication Type: Journal article

Publication year: 2026

Journal

International Journal of Magnetic Particle Imaging Infinite Science Publishing

Book Volume: 12

Article Number: 2603014

Journal Issue: 1

DOI: 10.18416/ijmpi.2026.2603014

Abstract

Rapid identification of WHO critical priority pathogens, such as Pseudomonas aeruginosa, is essential for managing sepsis, where standard blood cultures cause diagnostic delays of 24-72 hours. To address this, we developed a rapid, culture-independent magnetic immunoassay using a portable Magnetic Particle Spectroscopy (MPS) device for the direct detection of P. aeruginosa in blood. We evaluated two SPION formulations and found that our developed formulation, SPION-152, demonstrated excellent specificity, generating a strong signal change with virulent P. aeruginosa strains while showing negligible cross-reactivity with E. coli. Specificity for a virulence-associated factor was confirmed using a knockout mutant. These findings validate the MPS platform as a promising tool for accelerating pathogen identification in patients with suspected sepsis.

Authors with CRIS profile

Lukas Heinen Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christina Janko Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Stefan Lyer Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik)

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE) Universitätsklinikum Köln DE Germany (DE)

How to cite

APA:

Heinen, L., Simonis, A., Rybniker, J., Günther, J., Vogel, P., Janko, C.,... Tietze, R. (2026). Detection of Pseudomonas aeruginosa in Whole Blood using a portable Magnetic Particle Spectrometer-a feasibility study. International Journal of Magnetic Particle Imaging, 12(1). https://doi.org/10.18416/ijmpi.2026.2603014

MLA:

Heinen, Lukas, et al. "Detection of Pseudomonas aeruginosa in Whole Blood using a portable Magnetic Particle Spectrometer-a feasibility study." International Journal of Magnetic Particle Imaging 12.1 (2026).

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