Targeted gene delivery into α9β1-integrin-displaying cells by a synthetic peptide

Schneider H, Harbottle RP, Coutelle C, Yokosaki Y, Jost P (1999)

Publication Type: Journal article, Original article

Publication year: 1999

Journal

Febs Letters Elsevier

Book Volume: 458

Pages Range: 329-332

Journal Issue: 3

DOI: 10.1016/S0014-5793(99)01181-3

Abstract

We have investigated the usefulness of two small synthetic peptides comprising either a linear or a cyclic PLAEIDGIEL domain and a DNA-binding moiety of 16 lysine residues to mediate gene transfer selectively into α9β1-integrin-displaying cells. Such specific gene delivery could only be achieved with the peptide containing the cyclic PLAEIDGIEL domain. However, inclusion of the cationic liposome LipofectAMINE into the peptide/DNA complexes resulted for both peptides in efficient gene transfer with significant targeting specificity. Naturally, the integrin α9β1 is present only in a few highly specialised tissues and abundant throughout the human airway epithelia in vivo. Targeting gene vectors to this integrin therefore appears a useful approach to gene therapy of lung diseases such as cystic fibrosis. As the integrin α9β1 is associated with tissue differentiation during foetal development and may cause resurgence of the foetal phenotype in colon cancers, such vectors may also be applicable for prenatal and cancer gene therapy. Copyright (C) 1999 Federation of European Biochemical Societies.

Authors with CRIS profile

Holm Schneider

Involved external institutions

Imperial College London / The Imperial College of Science, Technology and Medicine GB United Kingdom (GB) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Schneider, H., Harbottle, R.P., Coutelle, C., Yokosaki, Y., & Jost, P. (1999). Targeted gene delivery into α9β1-integrin-displaying cells by a synthetic peptide. Febs Letters, 458(3), 329-332. https://doi.org/10.1016/S0014-5793(99)01181-3

MLA:

Schneider, Holm, et al. "Targeted gene delivery into α9β1-integrin-displaying cells by a synthetic peptide." Febs Letters 458.3 (1999): 329-332.

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