Factors influencing adenovirus-mediated airway transduction in fetal mice

Buckley SM, Waddington SN, Jezzard S, Lawrence L, Schneider H, Holder MV, Themis M, Coutelle C (2005)

Publication Language: English

Publication Type: Journal article, Original article

Publication year: 2005

Journal

Molecular Therapy Cell Press

Book Volume: 12

Pages Range: 484-492

Journal Issue: 3

DOI: 10.1016/j.ymthe.2005.02.020

Abstract

Intra-amniotic injection of adenovirus allows transduction of the fetal airways following natural fetal breathing movements. This administration method is promising for use in gene therapy for cystic fibrosis and other diseases for which the main target for exogenous gene expression is the lung. Here we have investigated factors that may affect the efficacy of gene transfer to the murine fetal lung. We examined marker compound distribution and transgene expression (from a first-generation adenoviral vector) at different stages of development. This demonstrated that fetal breathing movements at 15-16 days of gestation are of sufficient intensity to carry marker/vector into the fetal lungs. These movements can be significantly stimulated by the combination of intra-amniotic theophylline administration and postoperative exposure of the dam to elevated CO2 levels. However, the most important factor for efficient and consistent pulmonary transgene delivery is the dose of adenoviral vector used, as both the degree of transduction and the percentage of lungs transduced increases with escalating viral dose. Copyright © The American Society of Gene Therapy.

Authors with CRIS profile

Holm Schneider Department of Paediatrics and Adolescent Medicine

Involved external institutions

University College London (UCL) GB United Kingdom (GB)

How to cite

APA:

Buckley, S.M., Waddington, S.N., Jezzard, S., Lawrence, L., Schneider, H., Holder, M.V.,... Coutelle, C. (2005). Factors influencing adenovirus-mediated airway transduction in fetal mice. Molecular Therapy, 12(3), 484-492. https://doi.org/10.1016/j.ymthe.2005.02.020

MLA:

Buckley, Suzanne M.K., et al. "Factors influencing adenovirus-mediated airway transduction in fetal mice." Molecular Therapy 12.3 (2005): 484-492.

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