Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells

Amiri N, Schmid R, Schrüfer S, Lamberger Z, Stahlhut P, Lang G, Kulicke Y, Arkudas A, Horch RE, Müller-Seubert W (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Bioengineering MDPI

Book Volume: 13

Article Number: 25

Journal Issue: 1

DOI: 10.3390/bioengineering13010025

Abstract

Background: Reconstructive defect coverage after irradiation remains a challenge in reconstructive surgery, as ionizing radiation leads to tissue ischemia and fibrosis. Therefore, the application of adipose-derived stem cells (ASCs) might be a therapeutic strategy for improving flap survival. Nevertheless, the influence of irradiation on ASCs remains unclear. This study examines the effect of irradiation on 3D-printed ASCs. Methods: ASCs were 3D-cultured. The constructs were irradiated with 2 Gy and 5 Gy; one group treated with 0 Gy served as the non-irradiated control group. Cell viability was measured via a WST-8 assay, a live/dead assay and fluorescence microscopy 24 h, 48 h and 72 h after irradiation. Furthermore, qPCR analysis was performed to detect the expression of HIF-1α, p53 and IL-6 at the same timepoints. Results: Cell survival was high after 24 h. Expression of HIF1α after 24 h was 1.5 times significantly higher in the 2 Gy group compared with the 0 Gy group. The expression of other genes was not significantly affected by irradiation. Measurement of the metabolic activity and survival of the ASCs did not show differences between the different groups at all timepoints. Conclusions: 3D-cultured adipose-derived stem cells maintain high viability after moderate irradiation, suggesting radioresistance.

Authors with CRIS profile

Nicki Amiri Department of Plastic and Hand Surgery Rafael Schmid Department of Plastic and Hand Surgery Stefan Schrüfer Lehrstuhl für Werkstoffwissenschaften (Polymerwerkstoffe) (LSP) Yvonne Kulicke Department of Plastic and Hand Surgery Andreas Arkudas Medizinische Fakultät Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Wibke Müller-Seubert Medizinische Fakultät

Involved external institutions

Universitätsklinikum Würzburg DE Germany (DE)

How to cite

APA:

Amiri, N., Schmid, R., Schrüfer, S., Lamberger, Z., Stahlhut, P., Lang, G.,... Müller-Seubert, W. (2025). Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells. Bioengineering, 13(1). https://doi.org/10.3390/bioengineering13010025

MLA:

Amiri, Nicki, et al. "Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells." Bioengineering 13.1 (2025).

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