Lampersperger H, Tranchina M, Meth B, Han D, Nayebzadeh Eidgahi N, Reiter N, Kuth S, Lorke M, Boccaccini AR, Budday S, Karow M, Falk S (2025)
Publication Status: Submitted
Publication Type: Unpublished / Preprint
Future Publication Type: Journal article
Publication year: 2025
DOI: 10.1101/2025.01.08.631895
During neurodevelopment neural stem cells give rise to a spatially patterned tissue in which a regionally differentially regulated balance between proliferation and differentiation produces the fine-tuned number of neurons and macroglia necessary for a functional central nervous system. The cells driving these highly intricated developmental processes of patterning, growth and differentiation are constantly exposed to a mechanical environment that is however variable between different brain regions and along differentiation trajectories. Here we demonstrate that both, acute mechanical manipulations as well as a persistent change in the mechanical environment provided to human brain organoids, instruct neural stem cell lineage decisions. Furthermore, we dissect the underlying changes in the molecular program of organoid-resident cells by bulk- and single cell RNA-sequencing and reveal that mechanical manipulations impact on molecular programs governing early patterning events as well as cell-type specifically alter cellular metabolism. Thus, our results unravel a regulatory network linking mechanics and neural stem cell lineage decisions.
APA:
Lampersperger, H., Tranchina, M., Meth, B., Han, D., Nayebzadeh Eidgahi, N., Reiter, N.,... Falk, S. (2026). Mechanical impact on neural stem cell lineage decisions in human brain organoids. (Unpublished, Submitted).
MLA:
Lampersperger, Hanna, et al. Mechanical impact on neural stem cell lineage decisions in human brain organoids. Unpublished, Submitted. 2026.
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