Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses
Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S (2025)
Publication Type: Journal article
Publication year: 2025
Journal
DOI: 10.1016/j.devcel.2025.10.006
Abstract
Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.
Involved external institutions
Institute Of Photonic Sciences / Instituto de Ciencias Fotónicas / Institut de Ciències Fotòniques (ICFO-CERCA)
Spain (ES)
Centre for Genomic Regulation (CRG)
Spain (ES)
Fachhochschule Oberösterreich (FH OÖ) / University of Applied Sciences Upper Austria
Austria (AT)
Rheinische Friedrich-Wilhelms-Universität Bonn
Germany (DE)
Leiden University
Netherlands (NL)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
Institute of Science and Technology Austria (IST Austria)
Austria (AT)
Spain (ES)
Centre for Genomic Regulation (CRG)
Spain (ES)
Fachhochschule Oberösterreich (FH OÖ) / University of Applied Sciences Upper Austria
Austria (AT)
Rheinische Friedrich-Wilhelms-Universität Bonn
Germany (DE)
Leiden University
Netherlands (NL)
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
Institute of Science and Technology Austria (IST Austria)
Austria (AT)
How to cite
APA:
Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P.,... Wieser, S. (2025). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. https://doi.org/10.1016/j.devcel.2025.10.006
MLA:
Company-Garrido, Iván, et al. "Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses." Developmental Cell (2025).
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