Assessing Angiogenic Properties In Vitro or In Ovo of Pre-Crosslinked Alginate-Dialdehyde-Gelatin Hydrogels Incorporating Boron-Doped Mesoporous Bioactive Glass Particles

Schmid R, Kulicke Y, Sandor E, Lu HH, Fleischer S, Lang I, Wajant H, Kaňková H, Arkudas A, Kengelbach-Weigand A, Horch RE, Boccaccini AR, Promny T (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Advanced nanoBiomed research Wiley-VCH

DOI: 10.1002/anbr.202500143

Abstract

In this study, the influence of boron (B)-doped silicate-based 65%SiO2–0ÊO–15²O3 (mol% [15B]) mesoporous bioactive glass nanoparticles (MBGN) blended in cast and 3D printed pre-crosslinked alginate-dialdehyde-gelatin (ADA-GEL) constructs is examined, both in vitro and in ovo in the chick chorioallantoic membrane (CAM) model. Hydrogels such as ADA-GEL can be further enhanced by incorporating bioactive glasses (BGs) releasing ions targeting physiological pathways. The high biocompatibility, osteo- and angiogenic activity of boron-doped silicate-based BG compositions point to boron for the developing field of vascularized tissue engineering. The addition of 15B at 2% w/v leads to cell death in the case of human umbilical vein endothelial cells (HUVECs). Addition of 0.5% and 1% 15B does not change the sprouting of HUVEC spheroids in ADA-GEL. Interestingly, the main variability regarding HUVEC sprouting is shown between cast and 3D printed spheroids. Diffusion kinetics of ADA-GEL show no difference with and without MBGNS. The biocompatibility of 15B MBGNs is proved in ovo, however, no signs of neovascularization are observed. The integration with the CAM is demonstrated using a human embryonic kidney (HEK-293) reporter cell line. Taken together, it is concluded that modifying ADA-GEL with 15B does not improve its angiogenic characteristics in ovo, at concentrations <2%.

Authors with CRIS profile

Rafael Schmid Medizinische Fakultät Yvonne Kulicke Department of Plastic and Hand Surgery Evelin Sandor Department of Plastic and Hand Surgery Hsuan-Heng Lu Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Andreas Arkudas Medizinische Fakultät Annika Kengelbach-Weigand Department of Plastic and Hand Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien) Theresa Promny Department of Plastic and Hand Surgery

Involved external institutions

Universitätsklinikum Würzburg DE Germany (DE) Trenčianska univerzita Alexandra Dubčeka v Trenčíne SK Slovakia (SK)

How to cite

APA:

Schmid, R., Kulicke, Y., Sandor, E., Lu, H.-H., Fleischer, S., Lang, I.,... Promny, T. (2025). Assessing Angiogenic Properties In Vitro or In Ovo of Pre-Crosslinked Alginate-Dialdehyde-Gelatin Hydrogels Incorporating Boron-Doped Mesoporous Bioactive Glass Particles. Advanced nanoBiomed research. https://doi.org/10.1002/anbr.202500143

MLA:

Schmid, Rafael, et al. "Assessing Angiogenic Properties In Vitro or In Ovo of Pre-Crosslinked Alginate-Dialdehyde-Gelatin Hydrogels Incorporating Boron-Doped Mesoporous Bioactive Glass Particles." Advanced nanoBiomed research (2025).

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