Neutrophil Transmigration Analyses for Multiplexed Drug Screening and In Vivo Like Application Testing Using an Organ on a Chip Model

Reichardt C, Munoz Becerra L (2025)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2025

Journal

Methods in Molecular Biology Humana Press

Publisher: Humana Press Inc.

Edited Volumes: Autoimmunität. Methoden der Molekularbiologie

Series: Methods in Molecular Biology

Book Volume: 2990

Pages Range: 97-104

DOI: 10.1007/978-1-0716-4997-8_8

Abstract

Neutrophils are the most abundant type of innate immune cell in humans and make up for 40–60% of leukocytes in humans. As a part of the innate immunity and due to their high frequency, they form the first line of defense against harmful invaders. While neutrophils are crucial for combating infections, dysregulated neutrophil activity is a hallmark of chronic inflammation and autoimmune disorders. Here, we describe two methods to evaluate neutrophil migration, an in vitro transwell migration assay and an ex vivo organ-on-chip model, which allows quantification of the migration of neutrophils under flow and through human umbilical vein endothelial cells to best mimic conditions found in the human body.

Authors with CRIS profile

Clara Reichardt Department of Medicine 3 – Rheumatology and Immunology Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology

How to cite

APA:

Reichardt, C., & Munoz Becerra, L. (2026). Neutrophil Transmigration Analyses for Multiplexed Drug Screening and In Vivo Like Application Testing Using an Organ on a Chip Model. In Bechara, R. (Eds.), Autoimmunität. Methoden der Molekularbiologie. (pp. 97-104). Humana Press Inc..

MLA:

Reichardt, Clara, and Luis Munoz Becerra. "Neutrophil Transmigration Analyses for Multiplexed Drug Screening and In Vivo Like Application Testing Using an Organ on a Chip Model." Autoimmunität. Methoden der Molekularbiologie. Ed. Bechara, R., Humana Press Inc., 2026. 97-104.

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