Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview.

Delaby C, Ladang A, Martinez-Yriarte J, Zecca C, Logroscino G, Körtvelyessy P, Tumani H, Parchi P, Quadrio I, Hart M, Olsen DA, Alcolea D, Blennow K, Fortea J, Lleo A, Algeciras-Schimnich A, Ayrignac X, Bedel A, Santos GA, Borelli W, Bouaziz-Amar E, Baldeiras I, Bigot-Corbel E, Bjerke M, Carretero Perez M, Casoli T, Chabrashvili T, Chapman MD, Cusato J, Dursun E, Fourier A, Galimberti D, Gezen-Ak D, Gordon BA, Gouju J, de las Heras Florez S, Hernandez Sanchez J, Herwerth M, Imperiale D, Kaczorowski F, Kasuga K, Keshavan A, Khalil M, Kuhle J, Leithner C, Lewczuk P, Letournel F, Tsolaki M, Giuffrè GM, Blanc MC, Mroczko B, Martínez Rodríguez JE, Musso G, Kulczynska-Przybik A, Nogueira L, Paquet C, Baiardi S, Gaetani L, Parnetti L, Perez Garay R, Poesen K, Quillard-Muraine M, Rodriguez Borja E, Schraen S, Terracciano D, Bachhuber F, Halbgebauer S, Tzartos S, Tzartos J, Unterwalder N, Vermunt L, Wellington CL, Zetterberg H, Teunissen C, Lehmann S (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Book Volume: 21

Journal Issue: 6

DOI: 10.1002/alz.70343

Abstract

INTRODUCTION: Neurofilament light chain (NfL) quantification aids in diagnosing and predicting neurological disorders, but clinical and laboratory practices vary across centers. Differences in result interpretation and reporting further challenge test commutability. This study aimed to review the global analytical and post-analytical methods used for NfL measurement in routine clinical practice across different contexts. METHODS: We established an international working group (WG) and distributed a survey to its members to gather information on context of use (COU), (pre) analytical methods, cutoff usage, as well as the interpretation and reporting of NfL measurements. RESULTS: Among the centers, 63% measured NfL in cerebrospinal fluid (CSF), 87% in blood, and 53% in both. COU was widespread, with 50% defining pathological cutoffs based on publications and 42% considering age. Reporting was primarily done through numeric results (95%). DISCUSSION: Harmonizing cutoffs, reporting, and interpretation across various clinical contexts will facilitate the incorporation of this biomarker into routine clinical practice. HIGHLIGHTS: Unique international overview of current analytical and post-analytical methods for neurofilament light chain (NfL) measurement in routine clinical practice. Tailored sheets for each neurological application. Strategies to harmonize cutoffs, reporting, and interpretation of NfL's measurement.

Authors with CRIS profile

Involved external institutions

University College London (UCL) United Kingdom (GB) Mayo Clinic United States (USA) (US) Medizinische Universität Graz Austria (AT) Universitätsspital Basel Switzerland (CH) Charité - Universitätsmedizin Berlin Germany (DE) University of Montpellier / Université Montpellier France (FR) Centre hospitalier universitaire (CHU) d'Angers France (FR) University of Padua / Universita degli Studi di Padova Italy (IT) Università degli Studi di Napoli Federico II Italy (IT) Universität Ulm Germany (DE) Laboratori de Referència de Catalunya (LRC) Spain (ES) University of Bologna / Università di Bologna Italy (IT) Hospital Universitario Nuestra Señora de Candelaria (HUNSC) Spain (ES) Hospices Civils de Lyon (CHU) France (FR) Universitätsspital Zürich (USZ) Switzerland (CH) University of Turin / Università degli Studi di Torino (UNITO) Italy (IT) Odense Universitetshospital (OUH) Denmark (DK) Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven Belgium (BE) Hospital de la Santa Creu i Sant Pau Spain (ES) University of Gothenburg / Göteborgs universitet Sweden (SE) Hospital Center University Rouen / Centre hospitalier universitaire de Rouen (CHU) France (FR) Hospital Clínico Universitario de Valencia Spain (ES) Centre Hospitalier Régional Universitaire de Lille (CHRU de Lille) France (FR) Centre Hospitalier Universitaire de Bordeaux / CHU Bordeaux France (FR) AHEPA University General Hospital / Πανεπιστημιακό Νοσοκομείο ΑΧΕΠΑ Greece (GR) Catholic University of the Sacred Heart / Università Cattolica del Sacro Cuore Italy (IT) University of São Paulo / Universidade de São Paulo (USP) Brazil (BR) Hôpital Cochin France (FR) Universidade Federal do Rio Grande do Sul (UFRGS) Brazil (BR) Medical University of Bialystok Poland (PL) Hospital del Mar Spain (ES) Hospital Fernand Widal / Hôpital Fernand-Widal France (FR) Istanbul University Cerrahpaşa / İstanbul Üniversitesi Cerrahpaşa (IUC) Turkey (TR) Centre Hospitalier Universitaire de Liège (CHU Liège) Belgium (BE) Hospital do Meixoeiro Spain (ES) University of Bari Aldo Moro / Università degli Studi di Bari Aldo Moro Italy (IT) Washington University in St. Louis United States (USA) (US) Università degli studi di Milano Italy (IT) Niigata University / 新潟大学 Japan (JP) Osakidetza Spain (ES) Universitair Ziekenhuis Brussel Belgium (BE) Tzartos NeuroDiagnostics / Τζάρτος ΝευροΔιαγνωστική Greece (GR) Red Canaria de Laboratorios Spain (ES) National and Kapodistrian University of Athens Greece (GR) Istituto Nazionale di Riposo e Cura per Anziani (IRCCS INRCA) Italy (IT) Vivantes - Netzwerk für Gesundheit GmbH Germany (DE) Vrije Universiteit Amsterdam (VU) / University Amsterdam Netherlands (NL) State University of New York Upstate Medical University (SUNY) United States (USA) (US) University of British Columbia Canada (CA) National Hospital For Neurology and Neurosurgery United Kingdom (GB) Centre Hospitalier Universitaire (CHU) de Toulouse France (FR) Université Sorbonne Paris Cité France (FR) Universidade de Coimbra Portugal (PT) Università degli Studi di Perugia Italy (IT) Nantes University Hospital / Centre hospitalier universitaire de Nantes (CHU) France (FR)

How to cite

APA:

Delaby, C., Ladang, A., Martinez-Yriarte, J., Zecca, C., Logroscino, G., Körtvelyessy, P.,... Lehmann, S. (2025). Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview. Alzheimers & Dementia, 21(6). https://doi.org/10.1002/alz.70343

MLA:

Delaby, Constance, et al. "Clinical use and reporting of neurofilament quantification in neurological disorders: A global overview." Alzheimers & Dementia 21.6 (2025).

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