Neurofascin assembles a specialized extracellular matrix at the axon initial segment
Hedstrom KL, Xu X, Ogawa Y, Frischknecht R, Seidenbecher CI, Shrager P, Rasband MN (2007)
Publication Type: Journal article
Publication year: 2007
Journal
Book Volume: 178
Pages Range: 875-886
Journal Issue: 5
Abstract
Action potential initiation and propagation requires clustered Na + (voltage-gated Na+ [Nav]) channels at axon initial segments (AIS) and nodes of Ranvier. In addition to ion channels, these domains are characterized by cell adhesion molecules (CAMs; neurofascin-186 [NF-186] and neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. Schwann cells initiate peripheral nervous system node formation by clustering NF-186, which then recruits ankyrinG and Nav channels. However, AIS assembly of this protein complex does not require glial contact. To determine the AIS assembly mechanism, we silenced expres sion of AIS proteins by RNA interference. AnkyrinG knockdown prevented AIS localization of all other AIS proteins. Loss of NF-186, NrCAM, Nav channels, or βIV spectrin did not affect other neuronal AIS proteins. However, loss of NF-186 blocked assembly of the brevican-based AIS extracellular matrix, and NF-186 overexpression caused somatodendritic brevican clustering. Thus, NF-186 assembles and links the specialized brevican-containing AIS extracellular matrix to the intracellular cytoskeleton. © The Rockefeller University Press.
Authors with CRIS profile
Involved external institutions
University of Connecticut Health Center (UConn Health)
United States (USA) (US)
University of Rochester Medical Center Rochester, NY
United States (USA) (US)
Leibniz-Institut für Neurobiologie (LIN)
Germany (DE)
United States (USA) (US)
University of Rochester Medical Center Rochester, NY
United States (USA) (US)
Leibniz-Institut für Neurobiologie (LIN)
Germany (DE)
How to cite
APA:
Hedstrom, K.L., Xu, X., Ogawa, Y., Frischknecht, R., Seidenbecher, C.I., Shrager, P., & Rasband, M.N. (2007). Neurofascin assembles a specialized extracellular matrix at the axon initial segment. The Journal of Cell Biology, 178(5), 875-886. https://doi.org/10.1083/jcb.200705119
MLA:
Hedstrom, Kristian L., et al. "Neurofascin assembles a specialized extracellular matrix at the axon initial segment." The Journal of Cell Biology 178.5 (2007): 875-886.
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