Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging

Thoumine O, Ewers H, Heine M, Groc L, Frischknecht R, Giannone G, Poujol C, Legros P, Lounis B, Cognet L, Choquet D (2008)

Publication Type: Journal article, Review article

Publication year: 2008

Journal

Chemical Reviews American Chemical Society

Book Volume: 108

Pages Range: 1565-1587

Journal Issue: 5

DOI: 10.1021/cr078204m

Abstract

An overview on the various optical-based methods is given which shows the characterization of the assembly and turnover of macromolecular complexes that are present at neuronal contacts. The biological paradigm and some relevant molecular components has been explained while microscopy techniques have been described to show a dynamic visualization of specific elements at such contact sites. A focus is given specifically on the following: optical tweezers, FRAP, single-molecule and particle tracking, FCCS, and FRET/FLIM. Mathematical analysis were done on each case to present the physicochemical parameters that define protein-protein interactions.

Involved external institutions

Université de Bordeaux FR France (FR) National Center for Scientific Research / Centre national de la recherche scientifique (CNRS) FR France (FR) Leibniz-Institut für Neurobiologie (LIN) DE Germany (DE)

How to cite

APA:

Thoumine, O., Ewers, H., Heine, M., Groc, L., Frischknecht, R., Giannone, G.,... Choquet, D. (2008). Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging. Chemical Reviews, 108(5), 1565-1587. https://doi.org/10.1021/cr078204m

MLA:

Thoumine, Olivier, et al. "Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging." Chemical Reviews 108.5 (2008): 1565-1587.

BibTeX: Download