Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures

John N, Krügel H, Frischknecht R, Smalla KH, Schultz C, Kreutz MR, Gundelfinger ED, Seidenbecher CI (2006)

Publication Type: Journal article

Publication year: 2006

Journal

Molecular and Cellular Neuroscience Elsevier

Book Volume: 31

Pages Range: 774-784

Journal Issue: 4

DOI: 10.1016/j.mcn.2006.01.011

Abstract

Perineuronal nets (PNN) are specialized extracellular matrix structures enwrapping CNS neurons, which are important regulators for neuronal and synaptic functions. Brevican, a chondroitin sulfate proteoglycan, is an integral component of PNN. Here, we have investigated the appearance of these structures in hippocampal primary cultures. The expression profile of brevican in mixed cultures resembles the in vivo pattern with a strong upregulation of all isoforms during the second and 3rd weeks in culture. Brevican is primarily synthesized by co-cultured glial fibrillary acidic protein (GFAP-)-positive astrocytes and co-assembles with its interaction partners in PNN-like structures on neuronal somata and neurites as identified by counterstaining with the PNN marker Vicia villosa lectin. Both excitatory and inhibitory synapses are embedded into PNN. Furthermore, axon initial segments are strongly covered by a dense brevican coat. Altogether, we show that mature primary cultures can form PNN, and that basic features of these extracellular matrix structures may be studied in vitro. © 2006 Elsevier Inc. All rights reserved.

Authors with CRIS profile

Renato Frischknecht

Involved external institutions

Zentrum für neurowissenschaftliche Forschung / Center for Behavioral Brain Sciences (CBBS) DE Germany (DE) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Universitätsklinikum Magdeburg A.ö.R. DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE) Leibniz-Institut für Neurobiologie (LIN) DE Germany (DE) Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie (Hans-Knöll-Institut (HKI)) / Leibniz Institute for Natural Product Research and Infection Biology DE Germany (DE)

How to cite

APA:

John, N., Krügel, H., Frischknecht, R., Smalla, K.H., Schultz, C., Kreutz, M.R.,... Seidenbecher, C.I. (2006). Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures. Molecular and Cellular Neuroscience, 31(4), 774-784. https://doi.org/10.1016/j.mcn.2006.01.011

MLA:

John, Nora, et al. "Brevican-containing perineuronal nets of extracellular matrix in dissociated hippocampal primary cultures." Molecular and Cellular Neuroscience 31.4 (2006): 774-784.

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