LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis
Kupcinskas J, Steponaitiene R, Langner C, Smailyte G, Skieceviciene J, Kupcinskas L, Malfertheiner P, Link A (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 7
Article Number: 4828
Journal Issue: 1
DOI: 10.1038/s41598-017-05143-0
Abstract
LINE-1 hypomethylation is widely accepted as marker for global genomic DNA hypomethylation, which is a frequent event in cancer. The aim of the study was to evaluate LINE-1 methylation status at different stages of gastric carcinogenesis and evaluate its prognostic potential in clinical settings. LINE-1 methylation was analyzed in 267 tissue samples by bisulfite pyrosequencing including primary colorectal cancer tissues (T-CRC) with corresponding adjacent colon mucosa (N-CRC), gastric cancer tissues (T-GC) with corresponding gastric mucosa (N-GC), normal gastric tissues (N), chronic non-atrophic and atrophic gastritis (CG). LINE-1 methylation level was lower in both T-GC and T-CRC when compared to paired adjacent tissues. No difference was observed for LINE-1 methylation status between patients with normal gastric mucosa, CG and N-GC. LINE-1 methylation in T-GC but not N-GC tended to correlate with age. Subgroup stratification analysis did not reveal significant differences in LINE-1 methylation status according to tumor stage, anatomical location, histological subtype, differentiation grade. We observed similar overall survival data between patients with high or low LINE-1 levels. In summary, LINE-1 hypomethylation is a characteristic feature in GC but not very common in early preneoplastic stages of gastric carcinogenesis. Prognostic role of LINE-1 hypomethylation in GC patients could not be confirmed in this cohort.
Involved external institutions
Lithuanian University of Health Sciences / Lietuvos sveikatos mokslų universitetas (LSMU)
Lithuania (LT)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Lithuanian National Cancer Institute (NCI) / Nacionalinis vėžio institutas
Lithuania (LT)
Lithuania (LT)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
Lithuanian National Cancer Institute (NCI) / Nacionalinis vėžio institutas
Lithuania (LT)
How to cite
APA:
Kupcinskas, J., Steponaitiene, R., Langner, C., Smailyte, G., Skieceviciene, J., Kupcinskas, L.,... Link, A. (2017). LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-05143-0
MLA:
Kupcinskas, Juozas, et al. "LINE-1 hypomethylation is not a common event in preneoplastic stages of gastric carcinogenesis." Scientific Reports 7.1 (2017).
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