Food-Derived Xeno-microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof-of-Principle Study

Link J, Thon C, Schanze D, Steponaitiene R, Kupcinskas J, Zenker M, Canbay A, Malfertheiner P, Link A (2019)


Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 63

Article Number: 1800076

Journal Issue: 2

DOI: 10.1002/mnfr.201800076

Abstract

Scope: Diet is amongst the most crucial factors contributing to the multistep process of carcinogenesis. The role of exogenous microRNAs (miRNAs) is still debatable. In this proof-of-principle work, the presence of miRNAs in a variety of foods, its stability to processing, and detectability in GI mucosa and feces are studied and the effect of short-term diet on human- or plant-derived miRNAs in feces and blood is examined. Methods and results: Animal and plant miRNAs are detected in all foods irrespective of processing. Animal-derived foods showed the highest miRNA level and the lowest is found in cheese and milk. The impact of the short-term vegetarian or meat-rich diet on blood and feces miRNA is evaluated in healthy subjects using qPCR and Affymetrix profiling. Diet is not associated with changes in ultraconserved miRNAs. However, a vegetarian diet is associated with an increase of miR-168 in feces but not in blood. Overall, plant miR-168 is detectable in normal GI mucosa and in colorectal cancer. Conclusions: Food provides a great source of miRNAs and diet may be associated with changes in xenomiRs. Plant-derived miR-168 is ubiquitously present in feces, normal mucosa, and cancer. Further studies are needed to evaluate the functional interaction between diet-derived miRNAs and GI tract.

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How to cite

APA:

Link, J., Thon, C., Schanze, D., Steponaitiene, R., Kupcinskas, J., Zenker, M.,... Link, A. (2019). Food-Derived Xeno-microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof-of-Principle Study. Molecular Nutrition & Food Research, 63(2). https://doi.org/10.1002/mnfr.201800076

MLA:

Link, Jastin, et al. "Food-Derived Xeno-microRNAs: Influence of Diet and Detectability in Gastrointestinal Tract—Proof-of-Principle Study." Molecular Nutrition & Food Research 63.2 (2019).

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