Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study

Pinel-Cabello M, Jroundi F, López-Fernández M, Geffers R, Jarek M, Jauregui R, Link A, Vílchez-Vargas R, Merroun ML (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Hazardous Materials Elsevier

Book Volume: 403

Article Number: 123858

DOI: 10.1016/j.jhazmat.2020.123858

Abstract

The potential use of microorganisms in the bioremediation of U pollution has been extensively described. However, a lack of knowledge on molecular resistance mechanisms has become a challenge for the use of these technologies. We reported on the transcriptomic and microscopic response of Stenotrophomonas bentonitica BII-R7 exposed to 100 and 250 μM of U. Results showed that exposure to 100 μM displayed up-regulation of 185 and 148 genes during the lag and exponential phases, respectively, whereas 143 and 194 were down-regulated, out of 3786 genes (>1.5-fold change). Exposure to 250 μM of U showed up-regulation of 68 genes and down-regulation of 290 during the lag phase. Genes involved in cell wall and membrane protein synthesis, efflux systems and phosphatases were up-regulated under all conditions tested. Microscopic observations evidenced the formation of U-phosphate minerals at membrane and extracellular levels. Thus, a biphasic process is likely to occur: the increased cell wall would promote the biosorption of U to the cell surface and its precipitation as U-phosphate minerals enhanced by phosphatases. Transport systems would prevent U accumulation in the cytoplasm. These findings contribute to an understanding of how microbes cope with U toxicity, thus allowing for the development of efficient bioremediation strategies.

Involved external institutions

Universidad de Granada ES Spain (ES) Helmholtz-Zentrum Dresden-Rossendorf (HZDR) DE Germany (DE) Helmholtz-Zentrum für Infektionsforschung (HZI) DE Germany (DE) AgResearch NZ New Zealand (NZ) Otto-von-Guericke-Universität Magdeburg DE Germany (DE)

How to cite

APA:

Pinel-Cabello, M., Jroundi, F., López-Fernández, M., Geffers, R., Jarek, M., Jauregui, R.,... Merroun, M.L. (2021). Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study. Journal of Hazardous Materials, 403. https://doi.org/10.1016/j.jhazmat.2020.123858

MLA:

Pinel-Cabello, M., et al. "Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study." Journal of Hazardous Materials 403 (2021).

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