Profiling of the Bacterial Microbiota along the Murine Alimentary Tract

Vilchez-Vargas R, Salm F, Znalesniak EB, Haupenthal K, Schanze D, Zenker M, Link A, Hoffmann W (2022)

Publication Type: Journal article

Publication year: 2022

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 23

Article Number: 1783

Journal Issue: 3

DOI: 10.3390/ijms23031783

Abstract

Here, the spatial distribution of the bacterial flora along the murine alimentary tract was evaluated using high throughput sequencing in wild-type and Tff3-deficient (Tff3^KO) animals. Loss of Tff3 was linked to increased dextran sodium sulfate-induced colitis. This systematic study shows the results of 13 different regions from the esophagus to the rectum. The number of bacterial species (richness) increased from the esophagus to the rectum, from 50 to 200, respectively. Additionally, the bacterial community structure changed continuously; the highest changes were between the upper/middle and lower gastrointestinal compartments when comparing adjacent regions. Lactobacillus was the major colonizer in the upper/middle gastrointestinal tract, especially in the esophagus and stomach. From the caecum, a drastic diminution of Lactobacillus occurred, while members of Lachnospiraceae significantly increased. A significant change occurred in the bacterial community between the ascending and the transverse colon with Bacteroidetes being the major colonizers with relative constant abundance until the rectum. Interestingly, wild-type and Tff3^KO animals did not show significant differences in their bacterial communities, suggesting that Tff3 is not involved in alterations of intraluminal or adhesive microbiota but is obviously important for mucosal protec-tion, e.g., of the sensitive stem cells in the colonic crypts probably by a mucus plume.

Involved external institutions

Otto-von-Guericke-Universität Magdeburg

Germany (DE)

How to cite

APA:

Vilchez-Vargas, R., Salm, F., Znalesniak, E.B., Haupenthal, K., Schanze, D., Zenker, M.,... Hoffmann, W. (2022). Profiling of the Bacterial Microbiota along the Murine Alimentary Tract. International Journal of Molecular Sciences, 23(3). https://doi.org/10.3390/ijms23031783

MLA:

Vilchez-Vargas, Ramiro, et al. "Profiling of the Bacterial Microbiota along the Murine Alimentary Tract." International Journal of Molecular Sciences 23.3 (2022).

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