Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase activity

Kopic S, Corradini S, Sidani S, Murek M, Vardanyan A, Föller M, Ritter M, Geibel JP (2010)

Publication Type: Journal article

Publication year: 2010

Journal

Cellular Physiology and Biochemistry Karger

Book Volume: 25

Pages Range: 195-202

Journal Issue: 2-3

DOI: 10.1159/000276553

Abstract

The effects of ethanol on gastric acid secretion remain controversial. The present study examines the effect of low-dose (2%) short term (15-20min) ethanol exposure on gastric acid secretion via a potential interaction with AMP-activated protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide functional evidence for the interaction of ethanol and AMPK in modulating secretagogue-induced acid secretion. Individual rat gastric glands were loaded with the pH-sensitive dye BCECF and the secretagogues carbachol (200 μM) or histamine (200 μM) were added to induce secretion. Rates of pH recovery were calculated as ΔpH i /Δt. In one series of experiments, secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol was added in combination with compound C (20 μM), an AMPK inhibitor, to prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid secretion. In order to confirm modulation of AMPK activity by ethanol, the specific AMPK inhibitor compound C was used, which reversed the inhibitory effects of ethanol on stimulated acid secretion. This study demonstrates that low dose ethanol (2%) inhibits secretagogue-dependent acid secretion by activation of the AMPK pathway in rat gastric parietal cells. Copyright © 2010 S. Karger AG, Basel.

Involved external institutions

Yale University US United States (USA) (US) Paracelsus Medizinische Privatuniversität AT Austria (AT)

How to cite

APA:

Kopic, S., Corradini, S., Sidani, S., Murek, M., Vardanyan, A., Föller, M.,... Geibel, J.P. (2010). Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase activity. Cellular Physiology and Biochemistry, 25(2-3), 195-202. https://doi.org/10.1159/000276553

MLA:

Kopic, Sascha, et al. "Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase activity." Cellular Physiology and Biochemistry 25.2-3 (2010): 195-202.

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