B cell activating factor (Baff) is required for the development of intra-renal tertiary lymphoid organs in experimental kidney transplantation in rats
Steines L, Poth H, Herrmann M, Schuster A, Banas B, Bergler T (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 21
Pages Range: 1-14
Article Number: 8045
Journal Issue: 21
DOI: 10.3390/ijms21218045
Abstract
Intra-renal tertiary lymphoid organs (TLOs) are associated with worsened outcome in kidney transplantation (Ktx). We used an anti-BAFF (B cell activating factor) intervention to investigate whether BAFF is required for TLO formation in a full MHC-mismatch Ktx model in rats. Rats received either therapeutic immunosuppression (no rejection, NR) or subtherapeutic immunosuppression (chronic rejection, CR) and were sacrificed on d56. One group additionally received an anti-BAFF antibody (CR + AB). Intra-renal T (CD3+) and B (CD20+) cells, their proliferation (Ki67+), and IgG+ plasma cells were analyzed by immunofluorescence microscopy. Formation of T and B cell zones and TLOs was assessed. Intra-renal expression of TLO-promoting factors, molecules of T:B crosstalk, and B cell differentiation was analyzed by qPCR. Intra-renal B and T cell zones and TLOs were detected in CR and were associated with elevated intra-renal mRNA expression of TLO-promoting factors, including CXCL13, CCL19, lymphotoxin-β, and BAFF. Intra-renal plasma cells were also elevated in CR. Anti-BAFF treatment significantly decreased intra-renal B cell zones and TLO, as well as intra-renal B cell-derived TLO-promoting factors and B cell differentiation markers. We conclude that BAFF-dependent intra-renal B cells promote TLO formation and advance local adaptive alloimmune responses in chronic rejection.
Involved external institutions
Germany (DE)How to cite
APA:
Steines, L., Poth, H., Herrmann, M., Schuster, A., Banas, B., & Bergler, T. (2020). B cell activating factor (Baff) is required for the development of intra-renal tertiary lymphoid organs in experimental kidney transplantation in rats. International Journal of Molecular Sciences, 21(21), 1-14. https://doi.org/10.3390/ijms21218045
MLA:
Steines, Louisa, et al. "B cell activating factor (Baff) is required for the development of intra-renal tertiary lymphoid organs in experimental kidney transplantation in rats." International Journal of Molecular Sciences 21.21 (2020): 1-14.
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