Monitoring B cell alloresponses in rats
Steines L, Scharf M, Hoffmann P, Schuster A, Banas B, Bergler T (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 501
Article Number: 113212
DOI: 10.1016/j.jim.2021.113212
Abstract
Antibody-mediated rejection is a major cause of graft failure in organ transplantation. For this reason, B cell responses are of particular interest to transplantation research. Rats are important model organisms for transplant studies, but B cell alloimmune assays and B cell subset markers are poorly established in rats. We alloimmunized rats by donor blood injection using the high responder rat strain combination Brown Norway (donor) and Lewis (recipient) rats. Using splenocytes from alloimmunized and control rats, we established assays to assess allospecific B cell proliferation and the capacity to generate allospecific B memory cells and alloantibody-secreting cells after antigenic rechallenge in vitro using a mixed lymphocyte reaction. Furthermore, we defined a simple gating and sorting strategy for pre- and post-germinal center follicular B cells, as well as non-switched and switched plasmablasts. Our protocols for assessing B cell alloresponses and B cell subsets in rats may help to accelerate research into the role of B cells and manipulation of humoral alloresponses in transplant research.
Involved external institutions
Germany (DE)How to cite
APA:
Steines, L., Scharf, M., Hoffmann, P., Schuster, A., Banas, B., & Bergler, T. (2022). Monitoring B cell alloresponses in rats. Journal of Immunological Methods, 501. https://doi.org/10.1016/j.jim.2021.113212
MLA:
Steines, Louisa, et al. "Monitoring B cell alloresponses in rats." Journal of Immunological Methods 501 (2022).
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