CD163/CD63+ Monocyte-Derived DC Profiled in Tissue by Multi-Antigen Analysis (MAA) Discriminate Chronic Eczema and Psoriasis

Windorfer S, Kirr M, Fröhlich W, Plosnita B, Ostalecki C, Berking C, Sticherling M, Baur A (2025)

Publication Type: Journal article

Publication year: 2025

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 26

Article Number: 9077

Journal Issue: 18

DOI: 10.3390/ijms26189077

Abstract

Psoriasis (Pso) is a chronic inflammatory skin disease with a genetic predisposition and an assumed autoimmune pathomechanism. Autoantigens, dendritic cells (DCs), and the TNF/IL23/Il17 axis are seemingly the main drivers of this process. However, the difference to other DC-driven immune processes, like in eczematic skin, is insufficiently understood. Multi-antigen analysis (MAA) allows the staining of tissue with 100 antigens and more and provides a deeper insight into pathological processes, using advanced imaging analysis and quantification of topographical allocated processes. Here we used this technology to assess and compare the skin immune infiltrations in Pso, chronic eczema, and healthy controls. Tissue samples from both skin diseases (n = 30) were stained for 63 antigens, including 45 immune markers, and cells were analysed and quantified in both epidermis and dermis. The presence of different types of monocyte-derived DC in the epidermis was the most notable distinction between both skin diseases. While in Pso a monocyte-derived DC (CD14+CD1a+CD11c+) predominated, possibly a Langerhans cell (LC)-like DC, eczema displayed a marker combination of a seemingly more differentiated monocyte-derived DC (CD14+CD63+CD163+), potentially a DC3 cell type.

Authors with CRIS profile

Michael Kirr Institute of Clinical and Molecular Virology Carola Berking Lehrstuhl für Haut- und Geschlechtskrankheiten Michael Sticherling Professur für Dermatologie Andreas Baur Department of Dermatology

Involved external institutions

Bezirkskrankenhaus Passau DE Germany (DE) TissueGnostics AT Austria (AT)

How to cite

APA:

Windorfer, S., Kirr, M., Fröhlich, W., Plosnita, B., Ostalecki, C., Berking, C.,... Baur, A. (2025). CD163/CD63+ Monocyte-Derived DC Profiled in Tissue by Multi-Antigen Analysis (MAA) Discriminate Chronic Eczema and Psoriasis. International Journal of Molecular Sciences, 26(18). https://doi.org/10.3390/ijms26189077

MLA:

Windorfer, Sabrina, et al. "CD163/CD63+ Monocyte-Derived DC Profiled in Tissue by Multi-Antigen Analysis (MAA) Discriminate Chronic Eczema and Psoriasis." International Journal of Molecular Sciences 26.18 (2025).

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