Systematic discovery and engineering of synthetic immune receptors in plants

Ngou BPM, Wyler M, Schmid MW, Suzuki T, Albert M, Dohmae N, Kadota Y, Shirasu K (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Science American Association for the Advancement of Science

Book Volume: 389

Article Number: eadx2508

Journal Issue: 6764

DOI: 10.1126/science.adx2508

Abstract

plants deploy a diverse array of pattern recognition receptors (prrs), which perceive microbe-associated molecular patterns to activate immune responses. Leucine-rich repeat receptor-like kinase subgroup XII (Lrr-rLK-XII) represents one of the largest prr families owing to lineage-specific diversification. through bioinformatics and synthetic biology approaches, we characterized Lrr-rLK-XIIs from 285 plant species and identified a receptor, “SCOre,” that perceives cold shock protein (CSp) peptides. SCOre orthologs from multiple angiosperm lineages exhibit CSp recognition polymorphisms, indicating recurrent selection for pathogen recognition through substitutions at key amino acid residues. through functional phylogenomics and protein structure predictions, we engineered SCOre variants capable of detecting multiple phytopathogen CSp peptides, thus revealing the diverse prr recognition landscape in plants. Our strategy holds promise for engineering plant immune receptors, particularly for perennial crops.

Authors with CRIS profile

Markus Albert Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

RIKEN Center for Sustainable Resource Science (CSRS) / 環境資源科学研究センター JP Japan (JP)

How to cite

APA:

Ngou, B.P.M., Wyler, M., Schmid, M.W., Suzuki, T., Albert, M., Dohmae, N.,... Shirasu, K. (2025). Systematic discovery and engineering of synthetic immune receptors in plants. Science, 389(6764). https://doi.org/10.1126/science.adx2508

MLA:

Ngou, Bruno Pok Man, et al. "Systematic discovery and engineering of synthetic immune receptors in plants." Science 389.6764 (2025).

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