Neutrophil Heterogeneity Identifies an Association of LAMP1 With Proliferative Lupus Nephritis

Ostendorf L, Garantziotis P, Huang FY, Schett G, Lederer JA, Fava A, Rao DA, Grieshaber Bouyer R (2025)

Publication Type: Journal article

Publication year: 2025

Journal

European Journal of Immunology Wiley-VCH Verlag

Book Volume: 55

Article Number: e70022

Journal Issue: 8

DOI: 10.1002/eji.70022

Abstract

Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus (SLE) with limited biomarkers for early detection. While neutrophils contribute to SLE pathogenesis, their phenotypic heterogeneity in disease remains poorly characterized. Here, we used mass cytometry to profile blood neutrophils from patients with biopsy-confirmed proliferative LN and healthy controls. We identified a distinct population of activated neutrophils, marked by surface expression of lysosomal-associated membrane protein 1 (LAMP1/CD107a), that was virtually absent in healthy individuals. We demonstrate that LAMP1 resides intracellularly in resting neutrophils and translocates to the cell surface upon activation. Transcriptomic analysis revealed no difference in LAMP1 mRNA expression between patients with SLE and controls, confirming that surface LAMP1 reflects neutrophil activation rather than increased transcription. Soluble LAMP1 was significantly elevated in serum from patients with SLE compared with controls, with the highest levels in proliferative LN. In a large cohort of 225 patients with LN, urinary LAMP1 correlated with glomerular filtration rate, proteinuria, and histological activity indices. Together, our findings reveal LAMP1 as a marker of neutrophil activation in SLE and identify serum and urinary LAMP1 as potential noninvasive biomarkers for proliferative LN.

Authors with CRIS profile

Panagiotis Garantziotis Department of Medicine 3 – Rheumatology and Immunology Georg Schett Lehrstuhl für Innere Medizin III Ricardo Grieshaber Bouyer Professur für Klinische Systemimmunologie

Involved external institutions

Universitätsklinikum Heidelberg DE Germany (DE) Deutsches Rheuma-Forschungszentrum (DRFZ) DE Germany (DE) Harvard University US United States (USA) (US) Brigham and Women's Hospital (BWH) US United States (USA) (US) Johns Hopkins Hospital US United States (USA) (US)

How to cite

APA:

Ostendorf, L., Garantziotis, P., Huang, F.Y., Schett, G., Lederer, J.A., Fava, A.,... Grieshaber Bouyer, R. (2025). Neutrophil Heterogeneity Identifies an Association of LAMP1 With Proliferative Lupus Nephritis. European Journal of Immunology, 55(8). https://doi.org/10.1002/eji.70022

MLA:

Ostendorf, Lennard, et al. "Neutrophil Heterogeneity Identifies an Association of LAMP1 With Proliferative Lupus Nephritis." European Journal of Immunology 55.8 (2025).

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