Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites

Veldkamp CT, Kiermaier E, Gabel-Eissens SJ, Gillitzer ML, Lippner DR, DiSilvio FA, Mueller CJ, Wantuch PL, Chaffee GR, Famiglietti MW, Zgoba DM, Bailey AA, Bah Y, Engebretson SJ, Graupner DR, Lackner ER, LaRosa VD, Medeiros T, Olson ML, Phillips AJ, Pyles H, Richard AM, Schoeller SJ, Touzeau B, Williams LG, Sixt M, Peterson FC (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Biochemistry American Chemical Society

Book Volume: 54

Pages Range: 4163-4166

Journal Issue: 27

DOI: 10.1021/acs.biochem.5b00560

Abstract

CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

Involved external institutions

University of Wisconsin–Whitewater US United States (USA) (US) Institute of Science and Technology Austria (IST Austria) AT Austria (AT) Medical College of Wisconsin (MCW) US United States (USA) (US)

How to cite

APA:

Veldkamp, C.T., Kiermaier, E., Gabel-Eissens, S.J., Gillitzer, M.L., Lippner, D.R., DiSilvio, F.A.,... Peterson, F.C. (2015). Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites. Biochemistry, 54(27), 4163-4166. https://doi.org/10.1021/acs.biochem.5b00560

MLA:

Veldkamp, Christopher T., et al. "Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites." Biochemistry 54.27 (2015): 4163-4166.

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