Polysialylation controls dendritic cell trafficking by regulating chemokine recognition
Kiermaier E, Moussion C, Veldkamp CT, Gerardy-Schahn R, De Vries I, Williams LG, Chaffee GR, Phillips AJ, Freiberger F, Imre R, Taleski D, Payne RJ, Braun A, Förster R, Mechtler K, Mühlenhoff M, Volkman BF, Sixt M (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 351
Pages Range: 186-190
Journal Issue: 6269
Abstract
The addition of polysialic acid to N- and/or O-linked glycans, referred to as polysialylation, is a rare posttranslational modification that is mainly known to control the developmental plasticity of the nervous system. Here we show that CCR7, the central chemokine receptor controlling immune cell trafficking to secondary lymphatic organs, carries polysialic acid. This modification is essential for the recognition of the CCR7 ligand CCL21. As a consequence, dendritic cell trafficking is abrogated in polysialyltransferase-deficient mice, manifesting as disturbed lymph node homeostasis and unresponsiveness to inflammatory stimuli. Structure-function analysis of chemokine-receptor interactions reveals that CCL21 adopts an autoinhibited conformation, which is released upon interaction with polysialic acid. Thus, we describe a glycosylation-mediated immune cell trafficking disorder and its mechanistic basis.
Involved external institutions
Medizinische Hochschule Hannover (MHH) / Hannover Medical School
Germany (DE)
Institute of Science and Technology Austria (IST Austria)
Austria (AT)
University of Wisconsin–Whitewater
United States (USA) (US)
Forschungsinstitut für Molekulare Pathologie / Research Institute of Molecular Pathology (IMP)
Austria (AT)
Medical College of Wisconsin (MCW)
United States (USA) (US)
Germany (DE)
Institute of Science and Technology Austria (IST Austria)
Austria (AT)
University of Wisconsin–Whitewater
United States (USA) (US)
Forschungsinstitut für Molekulare Pathologie / Research Institute of Molecular Pathology (IMP)
Austria (AT)
Medical College of Wisconsin (MCW)
United States (USA) (US)
How to cite
APA:
Kiermaier, E., Moussion, C., Veldkamp, C.T., Gerardy-Schahn, R., De Vries, I., Williams, L.G.,... Sixt, M. (2016). Polysialylation controls dendritic cell trafficking by regulating chemokine recognition. Science, 351(6269), 186-190. https://doi.org/10.1126/science.aad0512
MLA:
Kiermaier, Eva, et al. "Polysialylation controls dendritic cell trafficking by regulating chemokine recognition." Science 351.6269 (2016): 186-190.
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