Bioengineered Bacterial Vesicles and Biomimetic Hybrids Eliminate Biofilms and Balance the Gut Microbiome

Pourtalebijahromi L, Kronast B, Munkert J, Sana L, Koch M, Danzer H, Dormeyer S, Jiang S, Herrmann F, Weiler M, Kashkanova A, Sandoghdar V, Zaiss M, Fuhrmann G (2025)

Publication Language: English

Publication Type: Journal article

Publication year: 2025

Journal

Small Wiley-VCH Verlag

DOI: 10.1002/smll.202502551

Abstract

Antibiotic-resistant pathogens are a global health challenge, necessitating innovative solutions beyond conventional antibiotics. This study introduces biomimetic nanocarriers - hybrids of bacteriomimetic liposomes and biocompatible Myxobacteria outer-membrane vesicles (OMVs) - as tunable platforms for targeted antibiotic delivery. Comparative analyses of their physicochemical properties and interactions with immune cells, intestinal epithelium, and biofilm-forming pathogens reveal distinct advantages. Hybrids excel at delivering antibiotics to intracellular targets, while Myxobacteria OMVs, particularly those of strain SBSr 073, evade immune clearance and prolong extracellular drug exposure. To support clinical translation, this study optimizes antibiotic encapsulation methods for SBSr 073 OMVs and evaluates the short- and long-term impact of Cystobacter ferrugineus 23 strain OMVs on the gut microbiome in mice. Summing up, this study highlights the promise of Myxobacteria OMVs and their biomimetic hybrids as versatile tools for treating Gram-negative biofilm-forming pathogens. These findings underscore the potential of bioengineered and biomimetic drug carriers for combating antimicrobial resistance and pave the way for their translation toward difficult-to-treat infections.

Authors with CRIS profile

Leila Pourtalebijahromi Lehrstuhl für Pharmazeutische Biologie Jennifer Munkert Lehrstuhl für Pharmazeutische Biologie Lorenzo Sana Lehrstuhl für Pharmazeutische Biologie Vahid Sandoghdar Lehrstuhl für Experimentalphysik Mario Zaiss Department of Medicine 3 – Rheumatology and Immunology Gregor Fuhrmann Lehrstuhl für Pharmazeutische Biologie

Involved external institutions

Leibniz-Institut für Neue Materialien gGmbH / Leibniz Institute for New Materials (INM) DE Germany (DE) Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE)

How to cite

APA:

Pourtalebijahromi, L., Kronast, B., Munkert, J., Sana, L., Koch, M., Danzer, H.,... Fuhrmann, G. (2025). Bioengineered Bacterial Vesicles and Biomimetic Hybrids Eliminate Biofilms and Balance the Gut Microbiome. Small. https://doi.org/10.1002/smll.202502551

MLA:

Pourtalebijahromi, Leila, et al. "Bioengineered Bacterial Vesicles and Biomimetic Hybrids Eliminate Biofilms and Balance the Gut Microbiome." Small (2025).

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