Cell-based ELISpot protocol to detect and quantify antigen-specific antibody-secreting cells in murine whole-organ single-cell suspensions

Brunner C, Menzel S, Rost F, Corte-Real J, Pracht K (2025)


Publication Language: English

Publication Type: Journal article

Publication year: 2025

Journal

Book Volume: 6

Article Number: 103797

Journal Issue: 3

DOI: 10.1016/j.xpro.2025.103797

Abstract

Antibody-secreting cells (ASCs) are the hallmark of the adaptive immune response to vaccines or infections. Here, we present a protocol to quantify the number of ASCs using an enzyme-linked immunospot assay (ELISpot). We describe the preparation of cell samples, ELISpot plates, and their quantification. This assay has potential applications in vaccine studies and research on the adaptive immune system or ASC-associated diseases. For complete details on the use and execution of this protocol please, refer to Bierling et al.1

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How to cite

APA:

Brunner, C., Menzel, S., Rost, F., Corte-Real, J., & Pracht, K. (2025). Cell-based ELISpot protocol to detect and quantify antigen-specific antibody-secreting cells in murine whole-organ single-cell suspensions. STAR Protocols, 6(3). https://doi.org/10.1016/j.xpro.2025.103797

MLA:

Brunner, Christina, et al. "Cell-based ELISpot protocol to detect and quantify antigen-specific antibody-secreting cells in murine whole-organ single-cell suspensions." STAR Protocols 6.3 (2025).

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