Continuous Stimulation with Glycolaldehyde-derived Advanced Glycation End Product Reduces Aggrecan and COL2A1 Production via RAGE in Human OUMS-27 Chondrosarcoma Cells
Hatipoglu OF, Nishinaka T, Yaykasli KO, Mori S, Watanabe M, Toyomura T, Nishibori M, Hirohata S, Takahashi H, Wake H (2025)
Publication Type: Journal article
Publication year: 2025
Journal
Book Volume: 79
Pages Range: 157-166
Journal Issue: 3
DOI: 10.18926/AMO/68723
Abstract
Chondrocytes are responsible for the production of extracellular matrix (ECM) components such as collagen type II alpha-1 (COL2A1) and aggrecan, which are loosely distributed in articular cartilage. Chondrocyte dysfunction has been implicated in the pathogenesis of rheumatic diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). With age, advanced glycation end products (AGEs) accumulate in all tissues and body fluids, including cartilage and synovial fluid, causing and accelerating pathological changes associated with chronic diseases such as OA. Glycolaldehyde-derived AGE (AGE3), which is toxic to a variety of cell types, have a stronger effect on cartilage compared with other AGEs. To understand the long-term effects of AGE3 on cartilage, we stimulated a human chondrosarcoma cell line (OUMS-27), which exhibits a chondrocytic phenotype, with 10 μg/ml AGE3 for 4 weeks. As a result, the expressions of COL2A1 and aggrecan were significantly downregulated in the OUMS-27 cells without inducing cell death, but the expressions of proteases that play an important role in cartilage destruction were not affected. Inhibition of the receptor for advanced glycation end products (RAGE) suppressed the AGE3-induced reduction in cartilage component production, suggesting the involvement of RAGE in the action of AGE3.
Authors with CRIS profile
Involved external institutions
Kindai University / 近畿大学
Japan (JP)
Shujitsu University / 就実大学
Japan (JP)
Okayama University / 岡山大学
Japan (JP)
Japan (JP)
Shujitsu University / 就実大学
Japan (JP)
Okayama University / 岡山大学
Japan (JP)
How to cite
APA:
Hatipoglu, O.F., Nishinaka, T., Yaykasli, K.O., Mori, S., Watanabe, M., Toyomura, T.,... Wake, H. (2025). Continuous Stimulation with Glycolaldehyde-derived Advanced Glycation End Product Reduces Aggrecan and COL2A1 Production via RAGE in Human OUMS-27 Chondrosarcoma Cells. Acta Medica Okayama, 79(3), 157-166. https://doi.org/10.18926/AMO/68723
MLA:
Hatipoglu, Omer Faruk, et al. "Continuous Stimulation with Glycolaldehyde-derived Advanced Glycation End Product Reduces Aggrecan and COL2A1 Production via RAGE in Human OUMS-27 Chondrosarcoma Cells." Acta Medica Okayama 79.3 (2025): 157-166.
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