Optimizing SPION Labeling for Single-Cell Magnetic Microscopy

Pointner A, Thalheim D, Belasi S, Heinen L, Bonato C, Luehmann T, Meijer J, Tietze R, Alexiou C, Schneider-Stock R, Nagy R (2025)

Publication Status: Submitted

Publication Type: Unpublished / Preprint

Future Publication Type: Journal article

Publication year: 2025

DOI: 10.48550/arXiv.2505.20373

Abstract

This study explores the correlation between iron mass on cell surfaces and the resultant magnetic field. Human colorectal cancer cells (HT29 line) were labeled with varying concentrations of SPIONs and imaged via a NV center widefield magnetic microscope. To assess the labeling efficacy, a convolutional neural network trained on simulated magnetic dipole data was utilized to reconstruct key labeling parameters on a cell-by-cell basis, including cell diameter, sensor proximity, and the iron mass associated with surface-bound SPIONs.

Our analysis provided quantitative metrics for these parameters across a range of labeling concentrations. The findings indicated that increasing SPION concentration enhances both the cell-surface iron mass and magnetic field strength, demonstrating a saturation effect. This methodology offers a coherent framework for the quantitative, high-throughput characterization of magnetically labeled cells, presenting significant implications for the fields of cell biology and magnetic sensing applications.

Authors with CRIS profile

Andre Pointner Lehrstuhl für Angewandte Quantentechnologien Daniela Thalheim Professur für Experimentelle Tumorpathologie Sarah Belasi Professur für Experimentelle Tumorpathologie Lukas Heinen Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Rainer Tietze Department of Otorhinolaryngology – Head and Neck Surgery (HNO-Klinik) Christoph Alexiou Professur für Nanomedizin Regine Schneider-Stock Professur für Experimentelle Tumorpathologie Roland Nagy Lehrstuhl für Angewandte Quantentechnologien

Involved external institutions

Felix Bloch Institute of Solid State Physics DE Germany (DE) Heriot-Watt University GB United Kingdom (GB)

How to cite

APA:

Pointner, A., Thalheim, D., Belasi, S., Heinen, L., Bonato, C., Luehmann, T.,... Nagy, R. (2025). Optimizing SPION Labeling for Single-Cell Magnetic Microscopy. (Unpublished, Submitted).

MLA:

Pointner, Andre, et al. Optimizing SPION Labeling for Single-Cell Magnetic Microscopy. Unpublished, Submitted. 2025.

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