Kinesin-12 POK2 polarization is a prerequisite for a fully functional division site and aids cell plate positioning
Livanos P, Kriechbaum C, Remers S, Herrmann A, Müller S (2025)
Publication Language: English
Publication Type: Journal article, Original article
Publication year: 2025
Journal
Book Volume: 16
Article Number: 4464
Journal Issue: 1
DOI: 10.1038/s41467-025-58852-w
Open Access Link: https://www.nature.com/articles/s41467-025-58852-w#article-info
Abstract
Division plane positioning in plants relies on the remarkably accurate selection of the cortical division zone (CDZ), earmarked by the preprophase band of microtubules. The PHRAGMOPLAST ORIENTING KINESIN 1 and 2 (POK1/2) play pivotal roles in CDZ maintenance in mitosis and guide the approaching phragmoplast during cytokinesis. Our knowledge regarding the mechanism of POK recruitment and subsequent maintenance is hitherto limited. Here, we report that POK2 arrives at the preprophase band utilizing a secondary microtubule-binding site within its carboxy-terminus. Effective tethering to the plasma membrane is achieved through non-specific electrostatic interactions with phospholipids. Reaching the cortex via premitotic cortical microtubules emerges as a prerequisite for POK2 polarization, as the order and the density of prophase microtubules become particularly relevant in the preprophase band-compromised mutant trm678. POK2 binding to cortical microtubules templates its future position at the cortex and appears critical for adequate division site setup and consequently tissue architecture.
Authors with CRIS profile
Panteleimon Livanos
Lehrstuhl für Zellbiologie
Choy Kriechbaum
Professur für Zelluläre Morphogenese
Sabine Müller
Professur für Zelluläre Morphogenese
Involved external institutions
Germany (DE)How to cite
APA:
Livanos, P., Kriechbaum, C., Remers, S., Herrmann, A., & Müller, S. (2025). Kinesin-12 POK2 polarization is a prerequisite for a fully functional division site and aids cell plate positioning. Nature Communications, 16(1). https://doi.org/10.1038/s41467-025-58852-w
MLA:
Livanos, Panteleimon, et al. "Kinesin-12 POK2 polarization is a prerequisite for a fully functional division site and aids cell plate positioning." Nature Communications 16.1 (2025).
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