Establishment of patient-derived 3D in vitro models of sarcomas: literature review and guidelines on behalf of the FORTRESS working group
De Cock L, Palubeckaitė I, Bersani F, Faehling T, Pasquali S, Umbaugh S, Meister MT, Danks MR, Manasterski P, Miallot R, Krumbholz M, Roohani S, Heymann D, Cidre-Aranaz F, Wozniak A, Schöffski P, Bovée JV, Merlini A, Venneker S (2025)
Publication Language: English
Publication Type: Journal article, Review article
Publication year: 2025
Journal
Book Volume: 65
Article Number: 101171
DOI: 10.1016/j.neo.2025.101171
Abstract
Sarcomas are a large family of rare and heterogeneous mesenchymal tumors, which respond poorly to available systemic treatments. Translation of preclinical findings into clinical applications has been slow, limiting improvements in patients’ outcomes and ultimately highlighting the need for a better understanding of sarcoma biology to develop more effective, subtype-specific therapies. To this end, reliable preclinical models are crucial, but the development of 3D in vitro sarcoma models has been lagging behind that of epithelial cancers. This is primarily due to the rarity and heterogeneity of sarcomas, and lack of widespread knowledge regarding the optimal growth conditions of these in vitro models. In this review, we provide an overview of currently available sarcoma tumoroid models, together with guidelines and suggestions for model development and characterization, on behalf of the FORTRESS (Forum For Translational Research in Sarcomas) international research working group on 3D sarcoma models.
Authors with CRIS profile
Involved external institutions
Katholieke Universiteit Leuven (KUL) / Catholic University of Leuven
Belgium (BE)
Leiden University Medical Center
Netherlands (NL)
University of Turin / Università degli Studi di Torino (UNITO)
Italy (IT)
Hopp-Kindertumorzentrum Heidelberg - KiTZ
Germany (DE)
Fondazione IRCCS: Istituto Nazionale dei Tumori
Italy (IT)
Universitätsklinikum Heidelberg
Germany (DE)
Princess Máxima Center
Netherlands (NL)
University of Edinburgh
United Kingdom (GB)
McGill University
Canada (CA)
Charité - Universitätsmedizin Berlin
Germany (DE)
Université de Nantes
France (FR)
Belgium (BE)
Leiden University Medical Center
Netherlands (NL)
University of Turin / Università degli Studi di Torino (UNITO)
Italy (IT)
Hopp-Kindertumorzentrum Heidelberg - KiTZ
Germany (DE)
Fondazione IRCCS: Istituto Nazionale dei Tumori
Italy (IT)
Universitätsklinikum Heidelberg
Germany (DE)
Princess Máxima Center
Netherlands (NL)
University of Edinburgh
United Kingdom (GB)
McGill University
Canada (CA)
Charité - Universitätsmedizin Berlin
Germany (DE)
Université de Nantes
France (FR)
How to cite
APA:
De Cock, L., Palubeckaitė, I., Bersani, F., Faehling, T., Pasquali, S., Umbaugh, S.,... Venneker, S. (2025). Establishment of patient-derived 3D in vitro models of sarcomas: literature review and guidelines on behalf of the FORTRESS working group. Neoplasia, 65. https://doi.org/10.1016/j.neo.2025.101171
MLA:
De Cock, Lore, et al. "Establishment of patient-derived 3D in vitro models of sarcomas: literature review and guidelines on behalf of the FORTRESS working group." Neoplasia 65 (2025).
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