Arkudas A, Pryymachuk G, Höreth T, Beier J, Polykandriotis E, Bleiziffer O, Horch RE, Kneser U (2009)
Publication Language: English
Publication Type: Journal article
Publication year: 2009
Book Volume: 15
Pages Range: 2501-2511
Journal Issue: 9
DOI: 10.1089/ten.tea.2008.0477
The angiogenic effects of different concentrations of vascular endothelial growth factor (VEGF) 165 and basic fibroblast growth factor (bFGF) immobilized in a fibrin-based drug-delivery system were quantitatively assessed in the arteriovenous (AV) loop model. An AV loop was created in the medial thigh of 60 rats. The loop was placed in a Teflon isolation chamber and embedded in 500 microL of fibrin gel loaded with VEGF and bFGF in four different concentrations (no growth factor, 100 ng/mL of VEGF, 25 ng/mL of VEGF and bFGF, 100 ng/mL pf VEGF and bFGF). The explantation intervals were 1, 2, and 4 weeks after the initial operation for all groups. Specimens were investigated using (micro-CT) and histological and morphometrical techniques. After 2 weeks, the cross-section area and construct weight were significantly lower with the use of 100 ng/mL of VEGF and bFGF. Micro-CT and histology showed significantly greater vascular density and number of vessels of the constructs at 2 and 4 weeks when 100 ng/mL of VEGF165 and bFGF were applied than in the growth factor-free specimens. The angioinductive effects were dose-dependent, with best results when using 100 ng/mL of VEGF165 and bFGF. The greater tissue formation was accompanied by faster resorption of the fibrin matrix.
APA:
Arkudas, A., Pryymachuk, G., Höreth, T., Beier, J., Polykandriotis, E., Bleiziffer, O.,... Kneser, U. (2009). Dose-finding study of fibrin gel-immobilized vascular endothelial growth factor 165 and basic fibroblast growth factor in the arteriovenous loop rat model. Tissue Engineering - Part A, 15(9), 2501-2511. https://doi.org/10.1089/ten.tea.2008.0477
MLA:
Arkudas, Andreas, et al. "Dose-finding study of fibrin gel-immobilized vascular endothelial growth factor 165 and basic fibroblast growth factor in the arteriovenous loop rat model." Tissue Engineering - Part A 15.9 (2009): 2501-2511.
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